Basic Vector Information
- Vector Name:
- yy630
- Antibiotic Resistance:
- Streptomycin
- Length:
- 9323 bp
- Type:
- LUC binary vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Yamamoto YY, Zhao CR.
- Promoter:
- NOS
yy630 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
yy630 vector Sequence
LOCUS 62056_23520 9323 bp DNA circular SYN 08-SEP-2020
DEFINITION LUC binary vector yy630 DNA, complete sequence.
ACCESSION LC578834
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9323)
AUTHORS Yamamoto YY, Zhao CR.
TITLE The yy630 vector was constructed by replacing the LUC+ gene in the
yy621 vector with the Nluc' gene of Promega pNL1.1[Nluc] vector. The
Nluc' gene is formed by replacing the initiation codon ATG of Nluc
gene with the ATGGCTATGGCT sequence
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9323)
AUTHORS Yamamoto YY, Zhao CR.
TITLE Direct Submission
JOURNAL Submitted (28-AUG-2020) Contact:Cheng-Ri Zhao Agricultural college
of Yanbian University, Department of horticulture & landscape
architecture; No.977, Gongyuan Road, Yanji, Jilin Province 133002,
China
REFERENCE 3 (bases 1 to 9323)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(28-AUG-2020) Contact:Cheng-Ri Zhao Agricultural college of Yanbian
University, Department of horticulture & landscape architecture;
No.977, Gongyuan Road, Yanji, Jilin Province 133002, China"
FEATURES Location/Qualifiers
source 1..9323
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 1187..1813
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 2250..3314
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 3383..3577
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 3921..4061
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(4247..4835)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5082..5870)
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
misc_feature 6398..6422
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
promoter 6576..6755
/label=NOS promoter
/note="nopaline synthase promoter"
misc_feature 6793..6848
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 6850..7398
/label=BlpR
/note="phosphinothricin acetyltransferase"
terminator 7428..8135
/label=OCS terminator
/note="octopine synthase terminator"
misc_feature 8148..8217
/label=CIP7 intron with 12 bp-deletion
/note="CIP7 intron with 12 bp-deletion"
promoter 8248..8294
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
misc_feature 8301..8306
/note="Recognition site of Bgl II restriction endonuclease"
CDS 8324..8833
/label=Nluc
/note="NanoLuc(R) luciferase"
terminator 8864..9117
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 9186..9210
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
This page is informational only.