pMXKDX-kanamycin-resistance-protein-gene vector (V016491)

LOCUS       V016491                 7318 bp    DNA     circular SYN 24-FEB-2021
DEFINITION  Exported.
ACCESSION   V016491
VERSION     V016491
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Novel Asaia sp. type II secretion signals for Plasmodium inhibition
            in Anopheles stephensi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2020) Biological Sciences, Duquesne University,
            600 Forbes Ave, Pittsburgh, PA 15219-3016, USA
REFERENCE   3  (bases 1 to 7318)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-OCT-2020) Biological Sciences, Duquesne University, 600 Forbes
            Ave, Pittsburgh, PA 15219-3016, USA"
FEATURES             Location/Qualifiers
     source          1..7318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1763
                     /label="PnptII"
                     /note="PnptII"
                     /regulatory_class="promoter"
     misc_feature    1767..1916
                     /label="pentapeptide MXKDX repeat protein secretion signal"
                     /note="pentapeptide MXKDX repeat protein secretion signal"
     CDS             1923..3272
                     /gene="phoA"
                     /label="Alkaline phosphatase"
                     /note="Alkaline phosphatase from Escherichia coli (strain
                     K12). Accession#: P00634"
     CDS             complement(4867..5526)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(5527..6296)
                     /direction=LEFT
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"