pAAV-EF1A-mScarlet-Gephyrin.FingR-IL2RGTC vector (V016470)

LOCUS       62056_2370        6963 bp DNA     circular SYN 27-JUN-2020
DEFINITION  Cloning vector pAAV-EF1A-mScarlet-Gephyrin.FingR-IL2RGTC, complete 
            sequence.
ACCESSION   MT586119
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Bensussen S, Shankar S, Ching KH, Zemel D, Ta TL, Mount RA, Shroff 
            SN, Gritton HJ, Fabris P, Vanbenschoten H, Beck C, Man H-Y., Han X.
  TITLE     A viral toolbox of genetically encoded fluorescent synaptic tags
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Bensussen S, Shankar S, Ching KH, Zemel D, Ta TL, Mount RA, Shroff 
            SN, Gritton HJ, Fabris P, Vanbenschoten H, Beck C, Man H-Y., Han X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Biomedical Engineering, Boston University, 
            44 Cummington Mall, Boston, MA 02215, USA
REFERENCE   3  (bases 1 to 6963)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JUN-2020) Biomedical Engineering, Boston University, 44 
            Cummington Mall, Boston, MA 02215, USA"
FEATURES             Location/Qualifiers
     source          1..6963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    228..249
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        264..294
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    379..564
                     /label=5' ITR
                     /note="5' ITR"
     misc_feature    575..586
                     /label=IL2RG binding site
                     /note="IL2RG binding site"
     promoter        598..1776
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     promoter        1793..1811
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1844..2539
                     /label=mScarlet-I
                     /note="bright monomeric red fluorescent protein evolved
                     from a synthetic template (Bindels et al., 2016)"
     CDS             2846..2869
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    2894..3417
                     /label=IL2RGTC
                     /note="IL2RGTC"
     misc_feature    3472..4060
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(4063..4079)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(4109..4230)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    4333..4522
                     /label=3' ITR
                     /note="3' ITR"
     primer_bind     complement(4540..4556)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4698..5153
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5179..5283
                     /label=AmpR promoter
     CDS             5284..6141
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6315..6903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"