precB5R.1 vector (V016462)

LOCUS       V016462                 4856 bp    DNA     circular SYN 02-APR-2020
DEFINITION  Exported.
ACCESSION   V016462
VERSION     V016462
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Omura N, Yoshikawa T, Fujii H, Shibamura M, Inagaki T, Kato H, Egawa
            K, Harada S, Yamada S, Takeyama H, Saijo M.
  TITLE     A novel system for constructing a recombinant highly-attenuated
            vaccinia virus strain (LC16m8) expressing foreign genes and its
            application for the generation of LC16m8-based vaccines against
            herpes simplex virus 2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Yoshikawa T, Saijo M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2017) Contact:Tomoki Yoshikawa National Institute
            of Infectious Diseases, Department of Virology I; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan URL
            :https://www.niid.go.jp/niid/en/
REFERENCE   3  (bases 1 to 4856)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-SEP-2017) Contact:Tomoki Yoshikawa National Institute of
            Infectious Diseases, Department of Virology I; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan URL
            :https://www.niid.go.jp/niid/en/"
FEATURES             Location/Qualifiers
     source          1..4856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        239..257
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    281..724
                     /function="Homologous recombination site around B5R in VAC"
                     /label="B5R upstream"
                     /note="B5R upstream"
     CDS             complement(754..1209)
                     /gene="gpt"
                     /label="Xanthine-guanine phosphoribosyltransferase"
                     /note="Xanthine-guanine phosphoribosyltransferase from
                     Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC).
                     Accession#: B7N8G9"
     regulatory      complement(1216..1255)
                     /label="VAC early late promoter"
                     /note="VAC early late promoter"
                     /regulatory_class="promoter"
     CDS             complement(1266..1973)
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      complement(1974..2013)
                     /label="VAC early late promoter"
                     /note="VAC early late promoter"
                     /regulatory_class="promoter"
     misc_feature    2035..2279
                     /function="Intramolecular homologous recombination site to
                     B5R upstream"
                     /label="B5R upstream (short)"
                     /note="B5R upstream (short)"
     regulatory      2319..2358
                     /label="VAC early late promoter"
                     /note="VAC early late promoter"
                     /regulatory_class="promoter"
     misc_feature    2359..2364
                     /label="BglII site for insertion of gene of interest"
                     /note="BglII site for insertion of gene of interest"
     regulatory      2364..2370
                     /label="Vac early termination sequence"
                     /note="Vac early termination sequence"
                     /regulatory_class="terminator"
     promoter        complement(2402..2420)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2427..2443)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    2543..3005
                     /function="Homologous recombination site around B5R in VAC"
                     /label="B5R downstream"
                     /note="B5R downstream"
     promoter        3042..3112
                     /label="AmpR promoter"
     CDS             3113..3970
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4087..4675
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"