pTrcHisA-xylB-(teF)xylC-pHIuorin vector (V016421)

LOCUS       V016421                 6809 bp    DNA     circular SYN 25-MAR-2019
DEFINITION  Exported.
ACCESSION   V016421
VERSION     V016421
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-DEC-2018) State Key Laboratory of Food Science and
            Technology, Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su
            214122, China
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (05-DEC-2018) State Key Laboratory of Food Science and Technology,
            Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 214122, China"
FEATURES             Location/Qualifiers
     source          1..6809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    263..332
                     /label="rrnG antiterminator"
                     /note="antiterminator from the E. coli rrnG leader region
                     (Berg et al., 1989)"
     CDS             383..406
                     /label="minicistron"
                     /note="synthetic cistron containing a ribosome binding site
                     (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
     CDS             425..442
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             446..478
                     /label="T7 tag (gene 10 leader)"
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             482..505
                     /label="Xpress(TM) tag"
                     /note="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
     CDS             521..1264
                     /gene="xylB"
                     /label="D-xylose 1-dehydrogenase"
                     /note="D-xylose 1-dehydrogenase from Caulobacter vibrioides
                     (strain ATCC 19089 / CB15). Accession#: Q9A9Z0"
     regulatory      1274..1285
                     /label="b0034 RBS"
                     /note="b0034 RBS"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    1304..1321
                     /label="TEVp cleave site"
                     /note="TEVp cleave site"
     misc_feature    1322..1342
                     /label="F degron"
                     /note="F degron"
     CDS             1343..2209
                     /gene="xylC"
                     /label="D-xylonolactone lactonase"
                     /note="D-xylonolactone lactonase from Caulobacter
                     vibrioides (strain ATCC 19089 / CB15). Accession#: Q9A9Z1"
     misc_feature    2219..2230
                     /label="B0034 RBS"
                     /note="B0034 RBS"
     CDS             2237..2950
                     /label="ratiometric pHluorin"
                     /note="pH-sensitive mutant of green fluorescent protein
                     (Miesenbock et al., 1998)"
     terminator      3162..3248
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3340..3367
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        3387..3478
                     /label="AmpR promoter"
     CDS             3479..4336
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4510..5098
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5284..5424)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     promoter        5610..5687
                     /label="lacI promoter"
     CDS             5688..6767
                     /label="lacI"
                     /note="lac repressor"