pMTL675555 vector (V016399)

LOCUS       V016399                 7997 bp    DNA     circular SYN 28-JUL-2021
DEFINITION  Exported.
ACCESSION   V016399
VERSION     V016399
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7997)
  AUTHORS   Lau MSH., Sheng L, Zhang Y, Minton NP.
  TITLE     Development of a Suite of Tools for Genome Editing in
            Parageobacillus thermoglucosidasius and Their Use to Identify the
            Potential of a Native Plasmid in the Generation of Stable Engineered
            Strains
  JOURNAL   ACS Synth Biol 10 (7), 1739-1749 (2021)
   PUBMED   34197093
REFERENCE   2  (bases 1 to 7997)
  AUTHORS   Lau MSH., Sheng L, Zhang Y, Minton NP.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2021) Biodiscovery Institute, School of Life
            Sciences, University of Nottingham, Science Road, University Park,
            Nottingham, Nottinghamshire NG7 2RD, United Kingdom
REFERENCE   3  (bases 1 to 7997)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol"; date: "2021"; volume: "10"; issue: "7"; pages: "1739-1749"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-MAY-2021) Biodiscovery Institute, School of Life Sciences,
            University of Nottingham, Science Road, University Park, Nottingham,
            Nottinghamshire NG7 2RD, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..7997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(12..4175)
                     /gene="cas9-2"
                     /label="CRISPR-associated endonuclease Cas9 2"
                     /note="CRISPR-associated endonuclease Cas9 2 from
                     Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9).
                     Accession#: Q03JI6"
     regulatory      complement(4176..4360)
                     /label="lactate dehydrogenase promoter and RBS"
                     /note="lactate dehydrogenase promoter and RBS"
                     /regulatory_class="promoter"
     regulatory      4367..4457
                     /label="glyceraldehyde 3-phosphate dehydrogenase promoter"
                     /note="glyceraldehyde 3-phosphate dehydrogenase promoter"
                     /regulatory_class="promoter"
     terminator      4595..4681
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4773..4800
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     primer_bind     complement(4810..4826)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5262..6263
                     /label="repB"
                     /note="RepB replication protein"
     CDS             6420..7190
                     /codon_start=1
                     /transl_table=11
                     /product="kanamycin nucleotidyltransferase"
                     /label="kanamycin nucleotidyltransferase"
                     /protein_id="QXV87314.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGR
                     QTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFYSEEILLDYASQVESDWPLTHG
                     QFFSILPIYDSGGYLEKVYQTAKSVEAQKFHDAICALIVEELFEYAGKWRNIRVQGPTT
                     FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSD
                     SEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     rep_origin      7198..7781
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"