pleCC vector (V016387)

LOCUS       62056_13780        7105 bp DNA     circular SYN 21-APR-2021
DEFINITION  Cloning vector pleCC, complete sequence.
ACCESSION   MW770351
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Li Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2021) Cell and Developmental Biology, University 
            of California, San Diego, 9500 Gilman Drive Tata Hall 5401, San 
            Diego, CA 92122, USA
REFERENCE   2  (bases 1 to 7105)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2021) Cell and Developmental Biology, University of 
            California, San Diego, 9500 Gilman Drive Tata Hall 5401, San Diego, 
            CA 92122, USA"
FEATURES             Location/Qualifiers
     source          1..7105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(127..715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(889..1746)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1747..1851)
                     /label=AmpR promoter
     CDS             3223..3897
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     CDS             3907..3927
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4068..4189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4192..4612
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3
                     snRNA (Port et al., 2014)"
     ncRNA           4612..4630
                     /label=guide_RNA
                     /note="ple Intron gRNA"
                     /ncRNA_class="guide_RNA"
     misc_RNA        4632..4707
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      4714..4941
                     /label=U6 terminal
                     /note="U6 terminal"
                     /regulatory_class="terminator"
     polyA_signal    complement(5007..5136)
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     CDS             complement(5154..5870)
                     /label=mCerulean
                     /note="enhanced monomeric variant of CFP (Rizzo et al.,
                     2004)"
     regulatory      6048..6091
                     /label=3XP3
                     /note="3XP3"
                     /regulatory_class="promoter"