pCMV-CytERM-mCherry-F30-2xPepper10 vector (V016380)

LOCUS       62056_6765        8130 bp DNA     circular SYN 10-SEP-2019
DEFINITION  Cloning vector pCMV-CytERM-mCherry-F30-2xPepper10, complete 
            sequence.
ACCESSION   MN052906
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Wu J, Zaccara S, Khuperkar D, Kim H, Tanenbaum ME, Jaffrey SR.
  TITLE     Live imaging of mRNA using RNA-stabilized fluorogenic proteins
  JOURNAL   Nat. Methods 16 (9), 862-865 (2019)
  PUBMED    31471614
REFERENCE   2  (bases 1 to 8130)
  AUTHORS   Wu J, Zaccara S, Khuperkar D, Kim H, Tanenbaum ME, Jaffrey SR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2019) Pharmacology, Weill Cornell Medicine, 1300 
            York Avenue, New York, NY 10022, United States
REFERENCE   3  (bases 1 to 8130)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2019"; volume: "16"; issue: "9"; pages: "862-865"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUN-2019) Pharmacology, Weill Cornell Medicine, 1300 York 
            Avenue, New York, NY 10022, United States"
FEATURES             Location/Qualifiers
     source          1..8130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        864..882
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1047..1751
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     gene            1761..3667
                     /gene="F30-2xPepper10"
                     /label=F30-2xPepper10
     3'UTR           1761..3667
                     /gene="F30-2xPepper10"
     polyA_signal    3727..3954
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4000..4428
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4442..4771
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4838..5629
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5806..5939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5976..5992)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6000..6016)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6024..6054)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6069..6090)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6378..6963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7137..7994)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7995..8099)
                     /label=AmpR promoter