Basic Vector Information
- Vector Name:
- pMRH13
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4476 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Hajimorad M, Gralnick JA.
pMRH13 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMRH13 vector Sequence
LOCUS 62056_17255 4476 bp DNA circular SYN 31-DEC-2020
DEFINITION Cloning vector pMRH13, complete sequence.
ACCESSION MG569793
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4476)
AUTHORS Hajimorad M, Gralnick JA.
TITLE Plasmid copy number characterization of a modular vector set in
Shewanella oneidensis MR-1 for synthetic biology applications
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4476)
AUTHORS Hajimorad M, Gralnick JA.
TITLE Direct Submission
JOURNAL Submitted (21-NOV-2017) Electrical and Computer Engineering,
California State University, Chico, 400 West First Street, Chico, CA
95929-0888, USA
REFERENCE 3 (bases 1 to 4476)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(21-NOV-2017) Electrical and Computer Engineering, California State
University, Chico, 400 West First Street, Chico, CA 95929-0888, USA"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..4476
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind complement(15..36)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(52..1131)
/label=lacI
/note="lac repressor"
promoter complement(1132..1209)
/label=lacI promoter
protein_bind 1502..1518
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
regulatory 1589..1608
/regulatory_class="ribosome_binding_site"
CDS 1609..2283
/label=mRFP1
/note="monomeric derivative of DsRed (Campbell et al.,
2002)"
terminator 2319..2390
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2406..2433
/label=T7Te terminator
/note="phage T7 early transcription terminator"
rep_origin 2510..3098
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
oriT complement(3297..3406)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
terminator complement(3421..3515)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(3549..4340)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
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