pAP114 vector (V016356)

LOCUS       V016356                 8513 bp    DNA     circular SYN 02-MAR-2019
DEFINITION  Exported.
ACCESSION   V016356
VERSION     V016356
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Muh U, Pannullo AG, Weiss DS, Ellermeier CD.
  TITLE     A xylose-inducible expression system and a CRISPRi-plasmid for
            targeted knock-down of gene expression in Clostridioides difficile
  JOURNAL   J. Bacteriol. (2019) In press
   PUBMED   30745377
REFERENCE   2  (bases 1 to 8513)
  AUTHORS   Muh U, Pannullo AG, Weiss DS, Ellermeier CD.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2019) Microbiology and Immunology, University of
            Iowa, 200-EMRB, 431 Newton Road, Iowa City, IA 52242, USA
REFERENCE   3  (bases 1 to 8513)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol. (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-JAN-2019) Microbiology and Immunology, University of Iowa,
            200-EMRB, 431 Newton Road, Iowa City, IA 52242, USA"
FEATURES             Location/Qualifiers
     source          1..8513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            complement(196..1422)
                     /gene="xylR"
                     /label="xylR"
     CDS             complement(196..1422)
                     /codon_start=1
                     /transl_table=11
                     /gene="xylR"
                     /product="XylR"
                     /label="xylR"
                     /note="xylose operon regulatory protein"
                     /protein_id="QBG38743.1"
                     /translation="MQNNLLFLCKKYYNIILRRNIKLVTDKYTIREMNERLVLEQIIKN
                     GPISRASIASTIGLNKATISAITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGI
                     SLSMDIGYDYIFSSLSYLDGTIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYK
                     VIGLTLAIHGITCENKVLFTPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFS
                     TVHNSLLSLSIHSGFGSGIIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQY
                     CSEKKVFEQLSSLENIPKIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAP
                     EIIYLNSQIISDIPEILQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNI
                     QNLTLINEI"
     CDS             1577..2284
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             complement(2634..3002)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(3035..3144)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(3523..4143)
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      complement(4506..5094)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     gene            complement(5887..7524)
                     /gene="repA"
                     /label="repA"
     CDS             complement(5887..7524)
                     /codon_start=1
                     /transl_table=11
                     /gene="repA"
                     /product="RepA"
                     /label="repA"
                     /note="replication protein"
                     /protein_id="QBG38746.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNSK
                     QLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINRYA
                     LGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVSEVT
                     NTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKKYCKD
                     VKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQVISVA
                     REWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIYSDEDTL
                     FNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITYKRKKKVK
                     NIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYTISYFVTMA
                     VIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQQRISNMIES
                     LLDTKILDVWETKINDRGFMYYTYRLNK"
     CDS             complement(7799..8311)
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /label="replication protein"
                     /note="OrfB"
                     /protein_id="QBG38747.1"
                     /translation="MRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNII
                     NDKLYYSNIAEFEEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNIT
                     KNGNTNYLKVKNKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSR
                     PSRVFLK"