Basic Vector Information
- Vector Name:
- pP-anti-VEGF-scFV-MS2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6366 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Han X, Yang J, Zeng F, Weng J
pP-anti-VEGF-scFV-MS2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pP-anti-VEGF-scFV-MS2 vector Sequence
LOCUS 62056_18320 6366 bp DNA circular SYN 26-MAY-2020
DEFINITION Cloning vector pP-anti-VEGF-scFV-MS2, complete sequence.
ACCESSION MN996870
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6366)
AUTHORS Han X, Yang J, Zeng F, Weng J, Zhang Y, Peng Q, Shen L, Ding S, Liu
K, Gao Y.
TITLE Programmable Synthetic Protein Circuits for the Identification and
Suppression of Hepatocellular Carcinoma
JOURNAL Mol Ther Oncolytics 17, 70-82 (2020)
PUBMED 32322664
REFERENCE 2 (bases 1 to 6366)
AUTHORS Liu K, Yang J, Ding S.
TITLE Direct Submission
JOURNAL Submitted (27-JAN-2020) Second Department of Hepatobiliary Surgery,
Zhujiang Hospital, No. 253, Industrial Avenue, Haizhu District,
Guangzhou, Guangdong 510245, China
REFERENCE 3 (bases 1 to 6366)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther
Oncolytics 17, 70-82 (2020)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(27-JAN-2020) Second Department of Hepatobiliary Surgery, Zhujiang
Hospital, No. 253, Industrial Avenue, Haizhu District, Guangzhou,
Guangdong 510245, China"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..6366
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 1685..1699
/codon_start=1
/label=enterokinase site
/note="enterokinase recognition and cleavage site"
/translation="DDDDK"
CDS 1730..1759
/codon_start=1
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
CDS 1781..1807
/codon_start=1
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
/translation="YPYDVPDYA"
misc_RNA 1843..1861
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
polyA_signal 2161..2385
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2431..2859
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2873..3202
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3269..3865
/codon_start=1
/label=PuroR
/note="puromycin N-acetyltransferase"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 4042..4175
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4212..4228)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4236..4252)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4260..4290)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4305..4326)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4614..5199)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5373..6230)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6231..6335)
/label=AmpR promoter
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