Basic Vector Information
- Vector Name:
- PB-Gal4-Teton-cis
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8224 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Chen B.
- Promoter:
- SV40
PB-Gal4-Teton-cis vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
PB-Gal4-Teton-cis vector Sequence
LOCUS 62056_3360 8224 bp DNA circular SYN 01-SEP-2019
DEFINITION Cloning vector PB-Gal4-Teton-cis, complete sequence.
ACCESSION MK422200
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8224)
AUTHORS Chen B.
TITLE Direct Submission
JOURNAL Submitted (17-JAN-2019) Stem Cell Center, Institute of Blood
REFERENCE 2 (bases 1 to 8224)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(17-JAN-2019) Stem Cell Center, Institute of Blood Transfusion,
Chinese Academy of Medical Sciences "
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..8224
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter complement(1..105)
/label=AmpR promoter
rep_origin complement(131..586)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 728..744
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1373..1391
/label=tet operator
/note="bacterial operator O2 for the tetR and tetA genes"
promoter 1581..1619
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 1684..2349
/label=CopGFP
/note="green fluorescent protein 2 from Pontellina plumata,
also known as ppluGFP2 (Shagin et al., 2004)"
CDS 2419..2472
/label=T2A
/note="2A peptide from Thosea asigna virus capsid protein"
regulatory 2529..2653
/note="UAS; upstream activating sequences"
/regulatory_class="transcriptional_cis_regulatory_region"
CDS 2970..3677
/label=mCherry
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
misc_feature 3678..3743
/note="P2A; 2A peptide"
polyA_signal 3792..4016
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
promoter 4068..4397
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 4445..4492
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 4511..4882
/label=BleoR
/note="antibiotic-binding protein"
polyA_signal 5015..5136
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6203..6219)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6227..6243)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6251..6281)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(6296..6317)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6605..7193)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7367..8224)
/label=AmpR
/note="beta-lactamase"
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