pET24a-G3-pM421-468-ELP_KV8F-79 vector (V016277)

LOCUS       62056_9845        6644 bp DNA     circular SYN 22-JUL-2019
DEFINITION  Cloning vector pET24a-G3-pM421-468-ELP_KV8F-79, complete sequence.
ACCESSION   MN082628
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Kim H, Gaynor AS, Chen W.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2019) Chemical and Biochemical Engineering, 
            University of Delaware, 150 Academy Street, Colburn Lab, Newark, DE 
            19716, USA
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-JUN-2019) Chemical and Biochemical Engineering, University of 
            Delaware, 150 Academy Street, Colburn Lab, Newark, DE 19716, USA"
FEATURES             Location/Qualifiers
     source          1..6644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(563..1375)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1497..2085
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2271..2413)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2518..2706)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(3481..3502)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3518..4597)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(4598..4675)
                     /label=lacI promoter
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5072..6478
                     /codon_start=1
                     /transl_table=11
                     /product="G3-pM421-468-ELP_KV8F-79"
                     /label=G3-pM421-468-ELP_KV8F-79
                     /protein_id="QDM54905.1"
                     /translation="MGGGLNLHLEEAYREGDNTYYRVNENYYPGASIYENERASRDSEF
                     QNEILKRELRRQACGRSKGPGVGVPGKGVPGVGVPGVGVPGVGVPGVGVPGFGVPGVGV
                     PGVGVPGVGVPGVGVPGKGVPGVGVPGVGVPGVGVPGVGVPGFGVPGVGVPGVGVPGVG
                     VPGVGVPGKGVPGVGVPGVGVPGVGVPGVGVPGFGVPGVGVPGVGVPGVGVPGVGVPGK
                     GVPGVGVPGVGVPGVGVPGVGVPGFGVPGVGVPGVGVPGVGVPGVGVPGKGVPGVGVPG
                     VGVPGVGVPGVGVPGFGVPGVGVPGVGVPGVGVPGVGVPGKGVPGVGVPGVGVPGVGVP
                     GVGVPGFGVPGVGVPGVGVPGVGVPGVGVPGKGVPGVGVPGVGVPGVGVPGVGVPGFGV
                     PGVGVPGVGVPGVGVPGVGVPGKGVPGVGVPGVGVPGVGVPGVGVPGFGVPGVGVPGVG
                     VPGVGVPGWP"
     CDS             6488..6505
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      6572..6619
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"