pCKD100 vector (V016274)

LOCUS       62056_6495        6205 bp DNA     circular SYN 03-NOV-2020
DEFINITION  Suicide vector pCKD100, complete sequence.
ACCESSION   MW039600
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Nazareno ES, Acharya B, Dumenyo CK.
  TITLE     A mini-Tn5-derived transposon with reportable and selectable markers
            enables rapid generation and screening of insertional mutants in 
            Gram-negative bacteria
  JOURNAL   Lett. Appl. Microbiol. (2020) In press
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Nazareno ES, Acharya B, Dumenyo CK.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 6205)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Lett. Appl.
            Microbiol. (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-SEP-2020) Agricultural "
FEATURES             Location/Qualifiers
     source          1..6205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   7..25
                     /label=Tn5 inverted repeat
                     /note="Tn5 inverted repeat"
     misc_feature    26..59
                     /label=rare-cutting endonuclease multiple cloning site I
                     /note="rare-cutting endonuclease multiple cloning site I"
     rep_origin      complement(84..472)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(610..1266)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     misc_feature    1341..1360
                     /label=rare-cutting endonuclease multiple cloning site II
                     /note="rare-cutting endonuclease multiple cloning site II"
     CDS             complement(1537..2328)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(2396..3109)
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
     gene            complement(3114..3165)
                     /gene="atpE"
                     /label=atpE
     regulatory      complement(3114..3165)
                     /gene="atpE"
                     /regulatory_class="ribosome_binding_site"
     repeat_region   3181..3198
                     /rpt_family="Tn5"
     oriT            3915..4024
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(4686..6113)
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"