pSH179 vector (V016254)

LOCUS       62056_19680        9436 bp DNA     circular SYN 31-AUG-2021
DEFINITION  Cloning vector pSH179, complete sequence.
ACCESSION   MT670352
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Hiekel SSH., Budhagatapalli NNB.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2020) Physiology and Cell Biology/Plant 
            Reproductive Biology, Leibniz Institute of Plant Genetics and Crop 
            Plant Research (IPK), Corrensstrasse 3, Gatersleben, Saxony-Anhalt 
            06466, Germany
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-JUN-2020) Physiology and Cell Biology/Plant Reproductive 
            Biology, Leibniz Institute of Plant Genetics and Crop Plant Research
            (IPK), Corrensstrasse 3, Gatersleben, Saxony-Anhalt 06466, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..25)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     regulatory      126..488
                     /label=TaU6p
                     /note="TaU6p"
                     /regulatory_class="promoter"
     misc_RNA        510..585
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    586..592
                     /label=poly-T signal
                     /note="poly-T signal"
     promoter        710..1385
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             1561..5661
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     terminator      5754..6006
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6015..6031)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6170..6344
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(6494..6518)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6797..6824)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(6916..7002)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(7171..7606)
                     /direction=LEFT
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     rep_origin      complement(7721..8309)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8402..9208)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"