Basic Vector Information
- Vector Name:
- pMAJIIc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6684 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Pena MM, Facincani AP, Ferreira H, Ferro JA.
- Promoter:
- araBAD
pMAJIIc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMAJIIc vector Sequence
LOCUS 62056_16475 6684 bp DNA circular SYN 29-JUN-2020
DEFINITION Cloning vector pMAJIIc, complete sequence.
ACCESSION MT119765
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6684)
AUTHORS Pena MM, Facincani AP, Ferreira H, Ferro JA.
TITLE Direct Submission
JOURNAL Submitted (26-FEB-2020) Departamento de Tecnologia, Univ. Estadual
Paulista 'Julio de Mesquita Filho' - UNESP - Jaboticabal, Via de
Acesso Prof.Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900,
Brazil
REFERENCE 2 (bases 1 to 6684)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(26-FEB-2020) Departamento de Tecnologia, Univ. Estadual Paulista
'Julio de Mesquita Filho' - UNESP - Jaboticabal, Via de Acesso
Prof.Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900, Brazil"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..6684
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 6..713
/label=mCherry
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
misc_feature complement(753..1561)
/label=Xac_amy
/note="Xac_amy"
primer_bind complement(1577..1593)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 1734..2162
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS 2506..3297
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
CDS 3318..4175
/label=AmpR
/note="beta-lactamase"
rep_origin 4349..4937
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5225..5246
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5261..5291
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5299..5315
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5323..5339
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(5447..6322)
/label=araC
/note="L-arabinose regulatory protein"
promoter 6349..6633
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
regulatory 6654..6659
/label=ribosome_binding_site
/note="ribosome_binding_site"
/regulatory_class="ribosome_binding_site"
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