pNRVL-caSAT1-ADE2RI vector (V016210)

LOCUS       62056_18210        8426 bp DNA     circular SYN 03-MAR-2020
DEFINITION  Expression vector pNRVL-caSAT1-ADE2RI, complete sequence.
ACCESSION   MT001913
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2020) Department of Microbiology, University of 
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-JAN-2020) Department of Microbiology, University of Szeged, 
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    570..575
                     /label=StuI site for cassette release
                     /note="StuI site for cassette release"
     misc_feature    576..1039
                     /note="site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     regulatory      1094..1591
                     /label=Candida albicans ACT1 promoter
                     /note="Candida albicans ACT1 promoter"
                     /regulatory_class="promoter"
     gene            1592..2818
                     /gene="caSAT1"
                     /label=caSAT1
     CDS             join(1592..1601,2256..2818)
                     /codon_start=1
                     /transl_table=11
                     /gene="caSAT1"
                     /product="streptothricin acetyltransferase"
                     /label=caSAT1
                     /note="codon-optimized for Candida albicans"
                     /protein_id="QID92300.1"
                     /translation="MDGEEVAALVIDNGSHMKISVIPEQVAETLDAENHFIVREVFDVH
                     LSDQGFELSTRSVSPYRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEH
                     IVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDL
                     FTYKTRPQVSNETAMYWYWFSGAQDDA"
     regulatory      2822..2951
                     /label=Candida albicans URA3 terminator
                     /note="Candida albicans URA3 terminator"
                     /regulatory_class="terminator"
     regulatory      complement(2985..3484)
                     /label=C. parapsilosis CPAR2_805940 (ADE2) terminator
                     /note="C. parapsilosis CPAR2_805940 (ADE2) terminator"
                     /regulatory_class="terminator"
     gene            complement(3485..5197)
                     /gene="ADE2"
                     /label=ADE2
                     /note="similar to ADE2 from Saccharomyces cerevisiae"
     CDS             complement(3485..5197)
                     /codon_start=1
                     /transl_table=11
                     /gene="ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase-like
                     protein"
                     /label=ADE2
                     /protein_id="QID92301.1"
                     /translation="MDSKTIGILGGGQLGRMIVEAAHRLNIKTVILDAPQSPAKQINAL
                     DEHVDGSFTDYESIAKLAEKVDILTVEIEHVDVDALKMIQLKFPSIEIYPLPDTIKLIQ
                     DKYLQKEHLIQHGIAVTDSQAIAENTTDNLLKIGQAYGYPYMLKSRTLAYDGRGNFVVK
                     DESYVEKALKFLADRPLYAEKWCPFSKELAVMVVRSIEGEVFSYPTVETIHQNNICHLV
                     YAPARINDTLAEKASILAQNAVKSFPGCGIFGVEMFLLESGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHVRAVTGLPMPRGFTEFSTTITNAIMLNVLGDKDTPNKELETCRRALE
                     TPHSTVYLYGKTTRPERKMGHINIVTSSMDDAENRLAYIMGKSDKLQASPDANGERQQP
                     LVGIIMGSDSDLPVMAVGARILKQFGVPFELTIVSAHRTPHRMSKYAIEAAQRGLKCII
                     AGAGGAAHLPGMVAAMTPLPVIGVPVKGSTLDGVDSLHSIVQMPRGIPVATVAINNSTN
                     AALLAVRILGAVDSKWLNKMTNYMQNMEEEVLKKAEVVEEIGYEDYLTDKLKK"
     CDS             complement(5800..5820)
                     /label=7xHis
                     /note="6xHis affinity tag"
     misc_feature    6204..6654
                     /note="site of recombination; CpNEUT5LDowndown from C. 
                     parapsilosis"
     misc_feature    6655..6660
                     /label=StuI site for cassette release
                     /note="StuI site for cassette release"
     promoter        complement(6668..6686)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6691..6707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6820..7626
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      7776..8364
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"