pGrna2 vector (V016198)

LOCUS       V016198                 7434 bp    DNA     circular SYN 17-MAY-2020
DEFINITION  Exported.
ACCESSION   V016198
VERSION     V016198
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Agarwal N.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2020) Vaccine and Infectious Disease Research
            Center, Translational Health Science and Technology Institute, 3rd
            Milestone, Gurgaon-Faridabad Expressway, NCR Biotech Science
            Cluster, Faridabad, Select One 121001, India
REFERENCE   2  (bases 1 to 7434)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2020) Vaccine and Infectious Disease Research Center,
            Translational Health Science and Technology Institute, 3rd
            Milestone, Gurgaon-Faridabad Expressway, NCR Biotech Science
            Cluster, Faridabad, Select One 121001, India"
FEATURES             Location/Qualifiers
     source          1..7434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(248..836)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             1197..2192
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     rep_origin      2462..4999
                     /label="oriM"
                     /note="oriM"
     primer_bind     5048..5064
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(5107..5125)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5130..5146)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(5217..5588)
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     promoter        complement(5607..5654)
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             complement(5811..6431)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     regulatory      6597..6904
                     /label="tetracycline-inducible promoter, Pmyc1TetO"
                     /note="tetracycline-inducible promoter, Pmyc1TetO"
                     /regulatory_class="promoter"
     misc_RNA        6923..6998
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     terminator      complement(7394..7421)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"