Basic Vector Information
- Vector Name:
- FUW-tetO
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9554 bp
- Type:
- Lentiviral transfer vector
- Replication origin:
- ori
- Source/Author:
- Kim J.
- Promoter:
- hPGK
FUW-tetO vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
FUW-tetO vector Sequence
LOCUS 62056_921 9554 bp DNA circular SYN 25-JAN-2019
DEFINITION Lentiviral transfer vector FUW-tetO, complete sequence.
ACCESSION MK318529
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9554)
AUTHORS Kim J.
TITLE Direct Submission
JOURNAL Submitted (18-DEC-2018) Biological Sciences, UCSD, 9500 Gilman Dr,
La Jolla, CA 92093, USA
REFERENCE 2 (bases 1 to 9554)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(18-DEC-2018) Biological Sciences, UCSD, 9500 Gilman Dr, La Jolla,
CA 92093, USA"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..9554
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 45..424
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 425..627
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 869..994
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1487..1720
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1905..1949
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 2098..2139
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 2247..2364
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2421..2691
/label=tetracycline response element
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2724..2762
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2862..2885
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
promoter 2891..3401
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
CDS 3423..4019
/label=PuroR
/note="puromycin N-acetyltransferase"
misc_feature 4053..4641
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4644..4660)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
LTR 5166..5346
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
polyA_signal 5378..5602
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5648..6076
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6090..6419
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 6467..6514
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 6533..6904
/label=BleoR
/note="antibiotic-binding protein"
polyA_signal 7037..7170
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7207..7223)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(7231..7247)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7255..7285)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(7300..7321)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(7609..8197)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8371..9228)
/label=AmpR
/note="beta-lactamase"
promoter complement(9229..9333)
/label=AmpR promoter
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