pDS210 vector (V016123)

LOCUS       62056_8590        5439 bp DNA     circular SYN 27-APR-2021
DEFINITION  Suicide vector pDS210, complete sequence.
ACCESSION   MW413896
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Susanti D, Frazier MC, Mukhopadhyay B.
  TITLE     A Genetic System for Methanocaldococcus jannaschii: An Evolutionary 
            Deeply Rooted Hyperthermophilic Methanarchaeon
  JOURNAL   Front Microbiol 10, 1256 (2019)
  PUBMED    31333590
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Khairunisa BH, Susanti D, Mukhopadhyay B.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-DEC-2020) Biochemistry, Virginia Tech, 340 West Campus
            Drive, Blacksburg, VA 24061, USA
REFERENCE   3  (bases 1 to 5439)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol 10, 1256 (2019)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-DEC-2020) Biochemistry, Virginia Tech, 340 West Campus Drive, 
            Blacksburg, VA 24061, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    665..666
                     /note="part of radA; downstream of fsr (mj_0870)"
     gene            667..1098
                     /gene="radA"
                     /locus_tag="mj_0869"
                     /label=radA
     CDS             667..1098
                     /codon_start=1
                     /transl_table=11
                     /gene="radA"
                     /locus_tag="mj_0869"
                     /product="RadA"
                     /label=radA
                     /note="downstream of fsr (mj_0870)"
                     /protein_id="QUC68720.1"
                     /translation="ARAYNSDMQMLYAENVENLIREGHNIKLVIVDSLTSTFRTEYIGR
                     GKLAERQQKLGRHMATLNKLADIYNCVVIVTNQVAARPDALFGPSEQAIGGHIVGHAAT
                     FRIFLRKAKGDKRVAKLYDSPHLPDAEAMFRITEKGIHD"
     misc_feature    1099..1164
                     /label=downstream of fsr (mj_0870)
                     /note="downstream of fsr (mj_0870)"
     misc_feature    1165..1172
                     /label=AscI restriction site
                     /note="AscI restriction site"
     gene            complement(1173..2390)
                     /gene="hmgA"
                     /locus_tag="mj_0705"
                     /label=hmgA
     CDS             complement(1173..2390)
                     /codon_start=1
                     /transl_table=11
                     /gene="hmgA"
                     /locus_tag="mj_0705"
                     /product="3-hydroxy-3-methylglutaryl coenzyme A reductase"
                     /label=hmgA
                     /protein_id="QUC68718.1"
                     /translation="MENYNDILEKMLNGEIKPYQLDKMFGSKIATEIRRKFIEKKVGIE
                     FKHICNYSIDEEMAMKKNIENMIGAIQIPLGFAGPLKINGEYAKGEFYIPLATTEGALV
                     ASVNRGCSIITKCGGATVRVIDDKMTRAPCLKTKSVVDAIKVRDWIRENFERIKEVAES
                     TTRHGKLIKIEPILIVGRNLYPRFVFKTGDAMGMNMVTIATEKACNFIEGELKKEGIFV
                     KTVAVSGNACVDKKPSGMNLINGRGKSIVAEVFLTEKEVNKYLKTTSQAIAEVNRLKNY
                     IGSAISNSMGFNAHYANIIGAIFLATGQDEAHIVEGSLGITMAEVEDDGLYFSVTLPDV
                     PIGTVGGGTRVETQKECLEMLGCYGDNKALKFAEIVGAAVLAGELSLLGALAAGHLGKA
                     HQELGR"
     regulatory      complement(2391..2706)
                     /note="Methanocaldococcus FS.406-22 S-layer promoter
                     (Psla)"
                     /regulatory_class="promoter"
     misc_feature    2707..2714
                     /label=AscI restriction site
                     /note="AscI restriction site"
     misc_feature    2715..2929
                     /label=upstream of fsr (mj_0870)
                     /note="upstream of fsr (mj_0870)"
     gene            2930..3214
                     /locus_tag="mj_0871"
                     /label=mj_0871
     CDS             2930..3214
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="mj_0871"
                     /product="hypothetical protein"
                     /label=mj_0871
                     /note="part of mj_0871; upstream of fsr (mj_0870)"
                     /protein_id="QUC68719.1"
                     /translation="MKISAYYTIRISIIVLTTVFIVNYIMSTGIMKKLSNMLSPILRRL
                     KVNPLSISSTLACFFSPTVGYSILAEGLKENKVNEREVIGASLANSFPSV"
     promoter        complement(3251..3269)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3290..3306)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3314..3330)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3338..3368)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3383..3404)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3692..4280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4454..5311)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5312..5416)
                     /label=AmpR promoter