pRS-LacZ vector (V016107)

LOCUS       V016107                 6526 bp    DNA     circular SYN 11-FEB-2020
DEFINITION  Exported.
ACCESSION   V016107
VERSION     V016107
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Swift RP, Rajaram K, Liu HB, Dziedzic A, Jedlicka AE, Roberts AD,
            Matthews KA, Jhun H, Bumpus NN, Tewari SG, Wallqvist A, Prigge ST.
  TITLE     A mevalonate bypass system facilitates elucidation of plastid
            biology in malaria parasites
  JOURNAL   PLoS Pathog. (2020) In press
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Prigge ST.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2019) Molecular Microbiology and Immunology, Johns
            Hopkins SPH, 615 N. Wolfe, rm E5132, Johns Hopkins SPH, Baltimore,
            MD 21205, USA
REFERENCE   3  (bases 1 to 6526)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS
            Pathog. (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-DEC-2019) Molecular Microbiology and Immunology, Johns Hopkins
            SPH, 615 N. Wolfe, rm E5132, Johns Hopkins SPH, Baltimore, MD 21205,
            USA"
FEATURES             Location/Qualifiers
     source          1..6526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_structure  9..30
                     /note="NotI LIC site; ligation-independent cloning"
     5'UTR           37..1018
                     /label="CAM promoter"
                     /note="CAM promoter"
     CDS             1025..1585
                     /gene="DHFR"
                     /label="Dihydrofolate reductase"
                     /note="Dihydrofolate reductase from Homo sapiens.
                     Accession#: P00374"
     3'UTR           1591..2155
                     /label="HRPII 3'"
                     /note="HRPII 3'"
     misc_structure  2165..2186
                     /note="NgoMIV LIC site; ligation-independent cloning"
     5'UTR           2191..3340
                     /label="U6 snRNA 5'"
                     /note="U6 snRNA 5'"
     misc_feature    3342..3347
                     /label="BsaI endonuclease site"
                     /note="BsaI endonuclease site"
     protein_bind    3354..3375
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3390..3420
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3428..3444
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3464..3637
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
     terminator      complement(3709..3752)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    3754..3759
                     /label="BsaI endonuclease site"
                     /note="BsaI endonuclease site"
     misc_RNA        3761..3836
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     3'UTR           3837..4343
                     /label="U6 snRNA 3'"
                     /note="U6 snRNA 3'"
     promoter        4628..4732
                     /label="AmpR promoter"
     CDS             4733..5590
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5764..6352
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"