Basic Vector Information
- Vector Name:
- pMJA219
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8065 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Wang R, Pscheid R, Ghumra A, Alcocer M.
- Promoter:
- CMV
pMJA219 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMJA219 vector Sequence
LOCUS 62056_16975 8065 bp DNA circular SYN 02-FEB-2019
DEFINITION Cloning vector pMJA219, complete sequence.
ACCESSION MH782476
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8065)
AUTHORS Wang R, Pscheid R, Ghumra A, Alcocer M.
TITLE The intrinsic allergenicity of nut proteins: a lipid story
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8065)
AUTHORS Wang R, Pscheid R, Ghumra A, Alcocer M.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2018) School of Biosciences, University of
Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire
LE12 5RD, United Kingdom
REFERENCE 3 (bases 1 to 8065)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(23-AUG-2018) School of Biosciences, University of Nottingham,
Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD,
United Kingdom"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..8065
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal complement(570..625)
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
CDS complement(782..1726)
/gene="TRB"
/label=TRB
/note="T cell receptor beta chain MC.7.G5 from Homo
sapiens. Accession#: P0DTU4"
promoter complement(1892..2095)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer complement(2096..2399)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
CDS 2714..3055
/gene="TRAV10"
/label=TRAV10
/note="T cell receptor alpha variable 10 from Homo sapiens.
Accession#: A0A0B4J240"
CDS 3122..3541
/gene="TRAC"
/label=TRAC
/note="T cell receptor alpha chain constant from Homo
sapiens. Accession#: P01848"
CDS 3563..3604
/label=V5 tag
/note="epitope tag from simian virus 5"
CDS 3614..3631
/label=6xHis
/note="6xHis affinity tag"
polyA_signal 3660..3884
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3930..4358
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4372..4701
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 4768..5559
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 5738..5871
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5908..5924)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5932..5948)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5956..5986)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(6001..6022)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6310..6898)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7072..7929)
/label=AmpR
/note="beta-lactamase"
promoter complement(7930..8034)
/label=AmpR promoter
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