pMJA219 vector (V016091)

LOCUS       V016091                 8065 bp    DNA     circular SYN 02-FEB-2019
DEFINITION  Exported.
ACCESSION   V016091
VERSION     V016091
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Wang R, Pscheid R, Ghumra A, Alcocer M.
  TITLE     The intrinsic allergenicity of nut proteins: a lipid story
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Wang R, Pscheid R, Ghumra A, Alcocer M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2018) School of Biosciences, University of
            Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire
            LE12 5RD, United Kingdom
REFERENCE   3  (bases 1 to 8065)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-AUG-2018) School of Biosciences, University of Nottingham,
            Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD,
            United Kingdom"
FEATURES             Location/Qualifiers
     source          1..8065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(570..625)
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     CDS             complement(782..1726)
                     /gene="TRB"
                     /label="T cell receptor beta chain MC.7.G5"
                     /note="T cell receptor beta chain MC.7.G5 from Homo
                     sapiens. Accession#: P0DTU4"
     promoter        complement(1892..2095)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2096..2399)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             2714..3055
                     /gene="TRAV10"
                     /label="T cell receptor alpha variable 10"
                     /note="T cell receptor alpha variable 10 from Homo sapiens.
                     Accession#: A0A0B4J240"
     CDS             3122..3541
                     /gene="TRAC"
                     /label="T cell receptor alpha chain constant"
                     /note="T cell receptor alpha chain constant from Homo
                     sapiens. Accession#: P01848"
     CDS             3563..3604
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             3614..3631
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    3660..3884
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3930..4358
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4372..4701
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4768..5559
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5738..5871
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5908..5924)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5932..5948)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5956..5986)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6001..6022)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6310..6898)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7072..7929)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7930..8034)
                     /label="AmpR promoter"