pBla_natural vector (V016087)

LOCUS       62056_4085        2697 bp DNA     circular SYN 16-JUN-2021
DEFINITION  Cloning vector pBla_natural, complete sequence.
ACCESSION   MW816822
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Hashimoto K, Fischer EC, Romesberg FE.
  TITLE     Efforts toward Further Integration of an Unnatural Base Pair into 
            the Biology of a Semisynthetic Organism
  JOURNAL   J Am Chem Soc (2021) In press
  PUBMED    34096294
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Hashimoto K.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2021) Department of Chemistry, The Scripps 
            Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 
            92037, USA
REFERENCE   3  (bases 1 to 2697)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-MAR-2021) Department of Chemistry, The Scripps Research 
            Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      109..654
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        807..911
                     /label=AmpR promoter
     RBS             926..948
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             957..1814
                     /label=AmpR
                     /note="beta-lactamase"
     misc_feature    1849..1884
                     /label=proK terminator
                     /note="proK terminator"
     promoter        1914..1944
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_feature    1956..1962
                     /label=5' leader from serT
                     /note="5' leader from serT"
     misc_feature    1963..2024
                     /label=tRNA Ser(UGA)
                     /note="tRNA Ser(UGA)"
     misc_feature    1977..1982
                     /label=BsaI
                     /note="BsaI"
     misc_feature    1983..1988
                     /label=KpnI
                     /note="KpnI"
     misc_feature    1989..1994
                     /label=BsaI
                     /note="BsaI"
     misc_feature    2025..2067
                     /label=3' UTR from serT
                     /note="3' UTR from serT"
     misc_feature    2068..2103
                     /label=proK terminator
                     /note="proK terminator"
     promoter        2151..2198
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2217..2588
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      2603..2697
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"