pSC101-P_bla_PtNTT2_Dead vector (V015991)

LOCUS       62056_19390        4839 bp DNA     circular SYN 16-JUN-2021
DEFINITION  Cloning vector pSC101-P_bla_PtNTT2_Dead, complete sequence.
ACCESSION   MW816819
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Hashimoto K, Fischer EC, Romesberg FE.
  TITLE     Efforts toward Further Integration of an Unnatural Base Pair into 
            the Biology of a Semisynthetic Organism
  JOURNAL   J Am Chem Soc (2021) In press
  PUBMED    34096294
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Hashimoto K.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2021) Department of Chemistry, The Scripps 
            Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 
            92037, USA
REFERENCE   3  (bases 1 to 4839)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-MAR-2021) Department of Chemistry, The Scripps Research 
            Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    71..184
                     /label=pUC19-like
                     /note="pUC19-like"
     regulatory      185..235
                     /regulatory_class="promoter"
     misc_feature    235
                     /label=TSS
                     /note="TSS"
     misc_feature    268..273
                     /label=Shine-Dalgarno
                     /note="Shine-Dalgarno"
     misc_feature    280..491
                     /note="similar to PtNTT2; PtNTT2"
     CDS             505..1815
                     /codon_start=1
                     /transl_table=11
                     /product="PtNTT2"
                     /label=PtNTT2
                     /protein_id="QWO78771.1"
                     /translation="MYGVLPAATAFIALYSKMSNAMGKKMLFYSTCIPFFTFFGLFDVF
                     IYPNAERLHPSLEAVQAILPGGAASGGMAVLAKIATHWTSALFYVMAEIYSSVSVGLLF
                     WQFANDVVNVDQAKRFYPLFAQMSGLAPVLAGQYVVRFASKAVNFEASMHRLTAAVTFA
                     GIMICIFYQLSSSYVERTESAKPAADNEQSIKPKKKKPKMSMVESGKFLASSQYLRLIA
                     MLVLGYGLSINFTEIMWKSLVKKQYPDPLDYQRFMGNFSSAVGLSTCIVIFFGVHVIRL
                     LGWKVGALATPGIMAILALPFFACILLGLDSPARLEIAVIFGTIQSLLSKTSKYALFDP
                     TTQMAYIPLDDESKVKGKAAIDVLGSRIGKSGGSLIQQGLVFVFGNIINAAPVVGVVYY
                     SVLVAWMSAAGRLSGLFQAQTEMDKADKMEAKTNKEK"
     terminator      1849..1943
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    1960..2065
                     /label=pCDF-1b-like
                     /note="pCDF-1b-like"
     CDS             complement(2166..2822)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2823..2925)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      3270..3492
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             3540..4487
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"