pMSx132 vector (V015968)

MSDS

Basic Vector Information

Vector Name:: pMSx132

Antibiotic Resistance:: Ampicillin

Length:: 5833 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Scheller L, Schmollack M, Bertschi A, Mansouri M

pMSx132 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMSx132 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_17380        5833 bp DNA     circular SYN 30-JUN-2020
DEFINITION  Cloning vector pMSx132, complete sequence.
ACCESSION   MT267329
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Scheller L, Schmollack M, Bertschi A, Mansouri M, Saxena P, 
            Fussenegger M.
  TITLE     Phosphoregulated orthogonal signal transduction in mammalian cells
  JOURNAL   Nat Commun 11 (1), 3085 (2020)
  PUBMED    32555187
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Scheller L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2020) STI IBI-STI LPDI, EPFL, AI 3123 (Batiment 
            AI), Station 19, Lausanne 1015, Switzerland
REFERENCE   3  (bases 1 to 5833)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "3085"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2020) STI IBI-STI LPDI, EPFL, AI 3123 (Batiment AI), Station
            19, Lausanne 1015, Switzerland"
FEATURES             Location/Qualifiers
     source          1..5833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    27..230
                     /label=SV40 early promoter
                     /note="SV40 early promoter"
     promoter        280..298
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             330..1385
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ-ORK"
                     /label=EnvZ-ORK
                     /protein_id="QKE44368.1"
                     /translation="MTSGSQVQLVESGGGLVQAGGSLRLSCTASGRTGTIYSMAWFRQA
                     PGKEREFLATVGWSSGITYYMDSVKGRFTISRDKGKNTVYLQMDSLKPEDTAVYYCTAT
                     RAYSVGYDYWGQGTQVTVSSASDDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAE
                     SINKDIEECNAIIEQFIDYLRTASMEMADLNAVLGEVIAAESGYEREIETALYPGSIEV
                     KMHPLSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQP
                     FVRGDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTK
                     EGASASGSTGV"
     misc_feature    339..701
                     /label=aCaffVHH
                     /note="aCaffVHH"
     misc_feature    420..443
                     /label=CDR1
                     /note="CDR1"
     misc_feature    492..515
                     /label=CDR2
                     /note="CDR2"
     misc_feature    633..668
                     /label=CDR3
                     /note="CDR3"
     misc_feature    708..1355
                     /note="EnvZ 232-450;GQEMP:AS"
     misc_feature    708..885
                     /label=envZ DHp
                     /note="envZ DHp"
     misc_feature    891..1355
                     /label=envZ CA
                     /note="envZ CA"
     polyA_signal    1422..1646
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1692..2120
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2134..2463
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2530..3321
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3498..3631
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3668..3684)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3692..3708)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3716..3746)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3761..3782)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4070..4655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4829..5686)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5687..5791)
                     /label=AmpR promoter

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