pNPTS138 vector (V015940)

LOCUS       62056_18190        5361 bp DNA     circular SYN 17-JUL-2019
DEFINITION  Cloning vector pNPTS138 plasmid pNPTS138, complete sequence.
ACCESSION   MK533795
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Ferro JA, Facincani AP, Tezza RD, Varani AM, Ferreira H.
  TITLE     The nucleotide sequence of the plasmid pNPTS138
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Ferro JA, Facincani AP, Tezza RD, Varani AM, Ferreira H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2019) Departamento de Tecnologia, Univ. Estadual 
            Paulista 'Julio de Mesquita Filho' - UNESP-Jaboticabal, Via de 
            Acesso Prof.Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900, 
            Brazil
REFERENCE   3  (bases 1 to 5361)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-FEB-2019) Departamento de Tecnologia, Univ. Estadual Paulista 
            'Julio de Mesquita Filho' - UNESP-Jaboticabal, Via de Acesso 
            Prof.Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900, Brazil"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
            The plasmid was a gift from Prof. Lucy Shapiro, Department of 
            Developmental Biology, Stanford University School of Medicine 
            Beckman Center 279 Campus Drive, B300 Stanford, CA 94305.
FEATURES             Location/Qualifiers
     source          1..5361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(158..174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(182..198)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(206..236)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(251..272)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(333..922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            1140..1249
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             1282..1650
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      1824..2275
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             2371..3183
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKFLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        3258..3685
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             3686..5104
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     5316..5332
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"