pJC14 vector (V015919)

LOCUS       V015919                 8625 bp    DNA     circular SYN 10-MAR-2019
DEFINITION  Exported.
ACCESSION   V015919
VERSION     V015919
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8625)
  AUTHORS   Cui J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) MCB, Dartmouth College, 14 Engineering
            Drive, Hanover, NH 03755, USA
REFERENCE   2  (bases 1 to 8625)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (28-JUN-2018) MCB, Dartmouth College, 14 Engineering Drive, Hanover,
            NH 03755, USA"
FEATURES             Location/Qualifiers
     source          1..8625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(100..1047)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(1095..1317)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     misc_feature    1844..2415
                     /label="upstream homology recombination flank"
                     /note="upstream homology recombination flank"
     CDS             2416..2994
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase gamma subunit"
                     /EC_number="1.2.7.1"
                     /label="pyruvate ferredoxin oxidoreductase gamma subunit"
                     /note="Clo1313_0020"
                     /protein_id="QBI89806.1"
                     /translation="MGKVVEIRWHGRGGQGAKTASLLLADAAFNTGKYIQGFPEYGPER
                     MGAPITAYNRISDEKLTIHSNIYEPDYVVVVDDTLLTSVDVTAGLKEDGAIIVNTPKTP
                     DEIRPLLKGYKGKVCTIDARKISIETLGKYFPNTPMLGAVVKVSKIMDEEEFLKDMVES
                     FKHKFANKPEVVEGNIKALERSMQEVKGL"
     CDS             2991..3296
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase delta subunit"
                     /EC_number="1.2.7.1"
                     /label="pyruvate ferredoxin oxidoreductase delta subunit"
                     /note="Clo1313_0021"
                     /protein_id="QBI89807.1"
                     /translation="MSKELRDVKPDVTWKEITSGGVIDSPGNAHLFKTGDWRSMKPVWN
                     EEKCKQCLLCNPVCPDSSIMVSEEGKMTGIDYDHCKGCGICSKVCPFKAIDFVEEV"
     CDS             3313..4497
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase alpha subunit"
                     /EC_number="1.2.7.1"
                     /label="pyruvate ferredoxin oxidoreductase alpha subunit"
                     /note="Clo1313_0022"
                     /protein_id="QBI89808.1"
                     /translation="MGIRERLSGNEATAIAMRQINPDVVAAFPITPSTEIPQYFSSYVA
                     DGLVDTEFVAVESEHSAMSACIGAQAAGARAMTATSANGLAYMWEALYIAASMRLPIVL
                     AAVNRALSGPINIHNDHSDTMGARDSGWIQLYSENNQEAYDNMLMAHRIGEHPDVMLPV
                     MVCQDGFITSHAIENIELVEDEKVKAFVGEYKPTHYLLDRENPISVGPLDLQMHYFEHK
                     RQQAQAMENAKKVILEVAEEFYKLTGRKYGFFEEYKTDDADVAIVVMNSTAGTVKYVID
                     EYRVKGKKVGLIKPRVFRPFPVDELAQALSKFKAVAVMDKADSFNAAGGPLFTEVTSAL
                     FTKGVFGPKVINYKFGLGGRDVKVDDIEVVCEKLLEIASTGKVDSVYNYLGVRE"
     CDS             4510..5445
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase beta subunit"
                     /EC_number="1.2.7.1"
                     /label="pyruvate ferredoxin oxidoreductase beta subunit"
                     /note="Clo1313_0023"
                     /protein_id="QBI89809.1"
                     /translation="MAYNLKEVAKKPERLTGGHRMCAGCGAPIVVRQVLKALKPEDHAV
                     ISAATGCLEVSTFIYPYTAWKDSFIHSAFENTGATISGAEAAYKVLKKKGKIEGETKFI
                     AFGGDGGTYDIGLQALSGAMERGHDMVYVCYDNGAYMNTGIQRSSATPKYADTTTSPVG
                     KKIPGKMQPRKDLTEVLVNHRIPYVAQTAPFGNMKDLYEKAEKAIYTPGPAFLNVLAPC
                     PRGWRYNTPDLMELSKLAVETCFWPLYEVIDGKYIINYKPKEKVPVKEFLKLQGRFKHL
                     FKAGNEYMLEEIQKEVDLRWERLLKLAGEA"
     misc_feature    5446..5988
                     /label="downstream homology recombination flank"
                     /note="downstream homology recombination flank"
     regulatory      5993..6523
                     /label="kanamycin promoter"
                     /note="kanamycin promoter"
                     /regulatory_class="promoter"
     gene            6524..7285
                     /gene="htk"
                     /label="htk"
     CDS             6524..7285
                     /codon_start=1
                     /transl_table=11
                     /gene="htk"
                     /product="highly thermostable kanamycin
                     nucleotidyltransferase"
                     /EC_number="2.7.7.46"
                     /label="htk"
                     /protein_id="QBI89810.1"
                     /translation="MKGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTD
                     GPYSDIEMMCVLSTEGVEFSYEWTTGEWKAEVNFYSEEILLDYASRVEPDWPLTHGRFF
                     SILPIYDPGGYFEKVYQTAKSVEAQKFHDAICALIVEELFEYAGKWRNIRVQGPTTFLP
                     SLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQPDLPPGYVQLCQLVMSGQLSDPEK
                     LLESLENFWNGVQEWAERHGYIVDVSKRIPF"
     CDS             7309..7887
                     /gene="tdk"
                     /label="Thymidine kinase"
                     /note="Thymidine kinase from Thermoanaerobacter
                     pseudethanolicus (strain ATCC 33223 / 39E). Accession#:
                     B0K7G9"
     misc_feature    7982..8625
                     /label="gene target internal homology recombination flank"
                     /note="gene target internal homology recombination flank"