Basic Vector Information
- Vector Name:
- 3xP3_attB_eGFP_Vasa-AcrIIA4
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7475 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Taxiarchi C, Beaghton A, Illansinhage Don N, Kyrou K
3xP3_attB_eGFP_Vasa-AcrIIA4 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
3xP3_attB_eGFP_Vasa-AcrIIA4 vector Sequence
LOCUS 62056_141 7475 bp DNA circular SYN 14-JUN-2021
DEFINITION Cloning vector 3xP3_attB_eGFP_Vasa-AcrIIA4, complete sequence.
ACCESSION MZ172909
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7475)
AUTHORS Taxiarchi C, Beaghton A, Illansinhage Don N, Kyrou K, Gribble M,
Shittu D, Collins SP, Beisel CL, Galizi R, Crisanti A.
TITLE A genetically encoded anti-CRISPR protein constrains gene drive
spread and prevents population suppression
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7475)
AUTHORS Taxiarchi C, Beaghton A, Illansinhage Don N, Kyrou K, Gribble M,
Shittu D, Collins SP, Beisel CL, Galizi R, Crisanti A.
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2021) Life Sciences, Imperial College London,
Exhibition Road, London SW7 2AZ, UK
REFERENCE 3 (bases 1 to 7475)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(10-MAY-2021) Life Sciences, Imperial College London, Exhibition
Road, London SW7 2AZ, UK"
FEATURES Location/Qualifiers
source 1..7475
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 869..1129
/label=3xp3
/note="3xp3"
/regulatory_class="promoter"
protein_bind 1135..1168
/label=attB
/note="minimal attB site for the phi-C31 integrase (Groth
et
al., 2000)"
CDS 1190..1906
/label=EGFP
/note="enhanced GFP"
polyA_signal 2032..2153
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2540..2644
/label=AmpR promoter
CDS 2645..3502
/label=AmpR
/note="beta-lactamase"
rep_origin 3676..4264
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4552..4573
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4588..4618
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4626..4642
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4650..4666
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
regulatory 4724..7014
/label=vasa2
/note="vasa2"
/regulatory_class="promoter"
CDS 7024..7044
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
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