pIIS18-BsaI vector (V015857)

LOCUS       62056_12850        2553 bp DNA     circular SYN 25-APR-2019
DEFINITION  Cloning vector pIIS18-BsaI DNA, complete sequence.
ACCESSION   LC459973
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Altaib H, Takiguchi Y, Kozakai T, Badr Y, Nomura I, Suzuki T.
  TITLE     A new E. coli entry vector series named pIIS18 for seamless cloning 
            using Type IIS restriction enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Altaib HE, Suzuki T.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2019) Contact:Hend Essam Altaib Gifu University, 
            Faculty of Applied Biological Sciences; 1-1 yanagido, Gifu, Gifu 
            prefecture 501-1193, Japan
REFERENCE   3  (bases 1 to 2553)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JAN-2019) Contact:Hend Essam Altaib Gifu University, Faculty of 
            Applied Biological Sciences; 1-1 yanagido, Gifu, Gifu prefecture 
            501-1193, Japan"
FEATURES             Location/Qualifiers
     source          1..2553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     294..310
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    314..373
                     /note="modified multiple cloning site to include two facing
                     TypeIIS cleavage sites overlapped by one blunt ended 
                     cleavage site."
     misc_feature    333..338
                     /label=BsaI cleavage site(TypeIIS cleavage site)
                     /note="BsaI cleavage site(TypeIIS cleavage site)"
     misc_feature    337..342
                     /note="Bsp68I cleavage site(Blunt end enzyme cleavage
                     site)"
     misc_feature    complement(341..346)
                     /gene="lacZ'"
                     /label=BsaI cleavage site(TypeIIS cleavage site)
                     /note="BsaI cleavage site(TypeIIS cleavage site)"
     primer_bind     complement(383..399)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(407..423)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(431..461)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(476..497)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(785..1373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1547..2404)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2405..2509)
                     /label=AmpR promoter