JS_segC_C vector (V015854)

LOCUS       V015854                 8444 bp    DNA     circular SYN 01-APR-2020
DEFINITION  Exported.
ACCESSION   V015854
VERSION     V015854
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Shin J.
  TITLE     Programming Escherichia coli to function as a digital display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Shin J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2020) Biomedical Engineering, Boston University,
            44 Cummington Mall, Boston, MA 02215, USA
REFERENCE   3  (bases 1 to 8444)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-JAN-2020) Biomedical Engineering, Boston University, 44
            Cummington Mall, Boston, MA 02215, USA"
FEATURES             Location/Qualifiers
     source          1..8444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(297..842)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(934..1746)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      1903..1984
                     /label="PSrpR"
                     /note="PSrpR"
                     /regulatory_class="promoter"
     regulatory      1985..2054
                     /label="PLux2"
                     /note="PLux2"
                     /regulatory_class="promoter"
     misc_feature    2055..2129
                     /label="RiboJ53"
                     /note="RiboJ53"
     regulatory      2130..2166
                     /label="P2"
                     /note="P2"
                     /regulatory_class="ribosome_binding_site"
     CDS             2167..2769
                     /codon_start=1
                     /transl_table=11
                     /product="PhlF"
                     /label="PhlF"
                     /protein_id="QIQ56079.1"
                     /translation="MARTPSRSSIGSLRSPHTHKAILTSTIEILKECGYSGLSIESVAR
                     RAGASKPTIYRWWTNKAALIAEVYENESEQVRKFPDLGSFKADLDFLLRNLWKVWRETI
                     CGEAFRCVIAEAQLDPATLTQLKDQFMERRREMPKKLVENAISNGELPKDTNRELLLDM
                     IFGFCWYRLLTEQLTVEQDIEEFTFLLINGVCPGTQR"
     regulatory      2770..2827
                     /label="ECK120033737"
                     /note="ECK120033737"
                     /regulatory_class="terminator"
     protein_bind    2852..2870
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2906..2980
                     /label="RiboJ10"
                     /note="RiboJ10"
     regulatory      2981..3018
                     /label="S2"
                     /note="S2"
                     /regulatory_class="ribosome_binding_site"
     CDS             3019..3660
                     /codon_start=1
                     /transl_table=11
                     /product="SrpR"
                     /label="SrpR"
                     /protein_id="QIQ56080.1"
                     /translation="MARKTAAEAEETRQRIIDAALEVFVAQGVSDATLDQIARKAGVTR
                     GAVYWHFNGKLEVLQAVLASRQHPLELDFTPDLGIERSWEAVVVAMLDAVHSPQSKQFS
                     EILIYQGLDESGLIHNRMVQASDRFLQYIHQVLRHAVTQGELPINLDLQTSIGVFKGLI
                     TGLLYEGLRSKDQQAQIIKVALGSFWALLREPPRFLLCEEAQIKQVKSFE"
     regulatory      3661..3750
                     /label="ECK120029600"
                     /note="ECK120029600"
                     /regulatory_class="terminator"
     regulatory      3755..3820
                     /label="PPhlF"
                     /note="PPhlF"
                     /regulatory_class="promoter"
     misc_feature    3821..3895
                     /label="RiboJ51"
                     /note="RiboJ51"
     regulatory      3896..3904
                     /label="H1"
                     /note="H1"
                     /regulatory_class="ribosome_binding_site"
     CDS             3905..4540
                     /codon_start=1
                     /transl_table=11
                     /product="HlyIIR"
                     /label="HlyIIR"
                     /protein_id="QIQ56081.1"
                     /translation="MKYILFEVCEMGKSREQTMENILKAAKKKFGERGYEGTSIQEITK
                     EAKVNVAMASYYFNGKENLYYEVFKKYGLANELPNFLEKNQFNPINALREYLTVFTTHI
                     KENPEIGTLAYEEIIKESARLEKIKPYFIGSFEQLKEILQEGEKQGVFHFFSINHTIHW
                     ITSIVLFPKFKKFIDSLGPNETNDTNHEWMPEDLVSRIISALTDKPNI"
     regulatory      4541..4600
                     /label="ECK120010799"
                     /note="ECK120010799"
                     /regulatory_class="terminator"
     regulatory      4647..4700
                     /label="PLacI"
                     /note="PLacI"
                     /regulatory_class="promoter"
     regulatory      4701..4728
                     /label="a part of PLacI"
                     /note="a part of PLacI"
                     /regulatory_class="ribosome_binding_site"
     CDS             4729..5808
                     /label="lacI"
                     /note="lac repressor"
     regulatory      5812..5829
                     /regulatory_class="ribosome_binding_site"
     CDS             5830..6450
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     regulatory      6454..6708
                     /regulatory_class="terminator"
     regulatory      6713..6747
                     /label="J23104"
                     /note="J23104"
                     /regulatory_class="promoter"
     regulatory      6748..6775
                     /regulatory_class="ribosome_binding_site"
     CDS             6776..7525
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     regulatory      7529..7556
                     /regulatory_class="ribosome_binding_site"
     CDS             7557..8312
                     /codon_start=1
                     /transl_table=11
                     /product="CinR"
                     /label="CinR"
                     /protein_id="QIQ56085.1"
                     /translation="MIENTYSEKFESAFEQIKAAANVDAAIRILQAEYNLDFVTYHLAQ
                     TIASKIDSPFVRTTYPDAWVSRYLLNCYVKVDPIIKQGFERQLPFDWSEVEPTPEAYAM
                     LVDAQKHGIDDNGYSIPVADKAQRRALLSLNAHIPADEWTELVRRCRNEWIEIAHLIHR
                     KAVYELHGENDPVPALSPREIECLHWTALGKDYKDISVILGISEHTTRDYLKTARFRLG
                     CTTISAAASRAVQLRIINPYRIRMTRRNW"
     terminator      8324..8395
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      8411..8438
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"