Basic Vector Information
- Vector Name:
- JS_segC_C
- Antibiotic Resistance:
- Kanamycin
- Length:
- 8444 bp
- Type:
- Cloning vector
- Replication origin:
- p15A ori
- Source/Author:
- Shin J.
JS_segC_C vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
JS_segC_C vector Sequence
LOCUS 62056_1360 8444 bp DNA circular SYN 01-APR-2020
DEFINITION Cloning vector JS_segC_C, complete sequence.
ACCESSION MN982374
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8444)
AUTHORS Shin J.
TITLE Programming Escherichia coli to function as a digital display
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8444)
AUTHORS Shin J.
TITLE Direct Submission
JOURNAL Submitted (19-JAN-2020) Biomedical Engineering, Boston University,
44 Cummington Mall, Boston, MA 02215, USA
REFERENCE 3 (bases 1 to 8444)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(19-JAN-2020) Biomedical Engineering, Boston University, 44
Cummington Mall, Boston, MA 02215, USA"
FEATURES Location/Qualifiers
source 1..8444
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(297..842)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
CDS complement(934..1746)
/label=KanR
/note="aminoglycoside phosphotransferase"
regulatory 1903..1984
/label=PSrpR
/note="PSrpR"
/regulatory_class="promoter"
regulatory 1985..2054
/label=PLux2
/note="PLux2"
/regulatory_class="promoter"
misc_feature 2055..2129
/label=RiboJ53
/note="RiboJ53"
regulatory 2130..2166
/label=P2
/note="P2"
/regulatory_class="ribosome_binding_site"
CDS 2167..2769
/codon_start=1
/transl_table=11
/product="PhlF"
/label=PhlF
/protein_id="QIQ56079.1"
/translation="MARTPSRSSIGSLRSPHTHKAILTSTIEILKECGYSGLSIESVAR
RAGASKPTIYRWWTNKAALIAEVYENESEQVRKFPDLGSFKADLDFLLRNLWKVWRETI
CGEAFRCVIAEAQLDPATLTQLKDQFMERRREMPKKLVENAISNGELPKDTNRELLLDM
IFGFCWYRLLTEQLTVEQDIEEFTFLLINGVCPGTQR"
regulatory 2770..2827
/label=ECK120033737
/note="ECK120033737"
/regulatory_class="terminator"
protein_bind 2852..2870
/label=tet operator
/note="bacterial operator O2 for the tetR and tetA genes"
misc_feature 2906..2980
/label=RiboJ10
/note="RiboJ10"
regulatory 2981..3018
/label=S2
/note="S2"
/regulatory_class="ribosome_binding_site"
CDS 3019..3660
/codon_start=1
/transl_table=11
/product="SrpR"
/label=SrpR
/protein_id="QIQ56080.1"
/translation="MARKTAAEAEETRQRIIDAALEVFVAQGVSDATLDQIARKAGVTR
GAVYWHFNGKLEVLQAVLASRQHPLELDFTPDLGIERSWEAVVVAMLDAVHSPQSKQFS
EILIYQGLDESGLIHNRMVQASDRFLQYIHQVLRHAVTQGELPINLDLQTSIGVFKGLI
TGLLYEGLRSKDQQAQIIKVALGSFWALLREPPRFLLCEEAQIKQVKSFE"
regulatory 3661..3750
/label=ECK120029600
/note="ECK120029600"
/regulatory_class="terminator"
regulatory 3755..3820
/label=PPhlF
/note="PPhlF"
/regulatory_class="promoter"
misc_feature 3821..3895
/label=RiboJ51
/note="RiboJ51"
regulatory 3896..3904
/label=H1
/note="H1"
/regulatory_class="ribosome_binding_site"
CDS 3905..4540
/codon_start=1
/transl_table=11
/product="HlyIIR"
/label=HlyIIR
/protein_id="QIQ56081.1"
/translation="MKYILFEVCEMGKSREQTMENILKAAKKKFGERGYEGTSIQEITK
EAKVNVAMASYYFNGKENLYYEVFKKYGLANELPNFLEKNQFNPINALREYLTVFTTHI
KENPEIGTLAYEEIIKESARLEKIKPYFIGSFEQLKEILQEGEKQGVFHFFSINHTIHW
ITSIVLFPKFKKFIDSLGPNETNDTNHEWMPEDLVSRIISALTDKPNI"
regulatory 4541..4600
/label=ECK120010799
/note="ECK120010799"
/regulatory_class="terminator"
regulatory 4647..4700
/label=PLacI
/note="PLacI"
/regulatory_class="promoter"
regulatory 4701..4728
/label=a part of PLacI
/note="a part of PLacI"
/regulatory_class="ribosome_binding_site"
CDS 4729..5808
/label=lacI
/note="lac repressor"
regulatory 5812..5829
/regulatory_class="ribosome_binding_site"
CDS 5830..6450
/label=TetR
/note="tetracycline repressor TetR"
regulatory 6454..6708
/regulatory_class="terminator"
regulatory 6713..6747
/label=J23104
/note="J23104"
/regulatory_class="promoter"
regulatory 6748..6775
/regulatory_class="ribosome_binding_site"
CDS 6776..7525
/gene="luxR"
/label=luxR
/note="Transcriptional activator protein LuxR from
Aliivibrio fischeri. Accession#: P12746"
regulatory 7529..7556
/regulatory_class="ribosome_binding_site"
CDS 7557..8312
/codon_start=1
/transl_table=11
/product="CinR"
/label=CinR
/protein_id="QIQ56085.1"
/translation="MIENTYSEKFESAFEQIKAAANVDAAIRILQAEYNLDFVTYHLAQ
TIASKIDSPFVRTTYPDAWVSRYLLNCYVKVDPIIKQGFERQLPFDWSEVEPTPEAYAM
LVDAQKHGIDDNGYSIPVADKAQRRALLSLNAHIPADEWTELVRRCRNEWIEIAHLIHR
KAVYELHGENDPVPALSPREIECLHWTALGKDYKDISVILGISEHTTRDYLKTARFRLG
CTTISAAASRAVQLRIINPYRIRMTRRNW"
terminator 8324..8395
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 8411..8438
/label=T7Te terminator
/note="phage T7 early transcription terminator"
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