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pPIsom-kanamycin-resistance-protein-gene vector (V015839)

Basic Vector Information

Vector Name:
pPIsom-kanamycin-resistance-protein-gene
Antibiotic Resistance:
Kanamycin
Length:
7318 bp
Type:
Cloning vector
Replication origin:
pBBR1 oriV
Source/Author:
Grogan CL, Lampe DJ.

pPIsom-kanamycin-resistance-protein-gene vector Vector Map

pPIsom-kanamycin-resistance-protein-gene7318 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300660069007200NeoR/KanRPnptIIpeptidyl-prolyl cis-trans isomerase secretion signalAlkaline phosphatasepBBR1 ReppBBR1 oriV

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pPIsom-kanamycin-resistance-protein-gene vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V015839                 7318 bp    DNA     circular SYN 24-FEB-2021
DEFINITION  Exported.
ACCESSION   V015839
VERSION     V015839
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Novel Asaia sp. type II secretion signals for Plasmodium inhibition
            in Anopheles stephensi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2020) Biological Sciences, Duquesne University,
            600 Forbes Ave, Pittsburgh, PA 15219-3016, USA
REFERENCE   3  (bases 1 to 7318)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-OCT-2020) Biological Sciences, Duquesne University, 600 Forbes
            Ave, Pittsburgh, PA 15219-3016, USA"
FEATURES             Location/Qualifiers
     source          1..7318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1763
                     /label="PnptII"
                     /note="PnptII"
                     /regulatory_class="promoter"
     misc_feature    1767..1916
                     /note="peptidyl-prolyl cis-trans isomerase secretion
                     signal"
     CDS             1923..3272
                     /gene="phoA"
                     /label="Alkaline phosphatase"
                     /note="Alkaline phosphatase from Escherichia coli (strain
                     K12). Accession#: P00634"
     CDS             complement(4867..5526)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(5527..6296)
                     /direction=LEFT
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"

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