pAH68-AF_SNS126 vector (V015837)

LOCUS       V015837                 5167 bp    DNA     circular SYN 22-MAY-2021
DEFINITION  Exported.
ACCESSION   V015837
VERSION     V015837
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Foster AD, Chung C-W., Hann MM, Simpson GL, Tavassoli A.
  TITLE     Development of a fluorescent three-hybrid system for the
            identification of protein-protein associators
  JOURNAL   Pept Sci (Hoboken) 113 (1), e24178 (2021)
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Foster AD.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2020) Chemistry, University of Southampton,
            Building 27, University Road, Southampton SO17 1BJ, United Kingdom
REFERENCE   3  (bases 1 to 5167)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pept Sci
            (Hoboken)"; date: "2021"; volume: "113"; issue: "1"; pages: "e24178"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-MAY-2020) Chemistry, University of Southampton, Building 27,
            University Road, Southampton SO17 1BJ, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..5167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..79
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             80..1159
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    1175..1196
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1405..1433
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1441..1457
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1839..2159
                     /label="FKBP"
                     /note="human FK506-binding protein FKBP12"
     CDS             2187..2471
                     /gene="CI"
                     /label="Repressor protein CI"
                     /note="Repressor protein CI from Enterobacteria phage 434.
                     Accession#: P16117"
     CDS             2508..2783
                     /label="FRB"
                     /note="FKBP-rapamycin binding domain of human FRAP"
     terminator      2844..3090
                     /label="lambda tL3 terminator"
                     /note="transcription terminator tL3 from phage lambda"
     protein_bind    3125..3351
                     /label="HK022 attP"
                     /note="attachment site of phage HK022"
     rep_origin      complement(3385..3773)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(3878..4735)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4736..4840)
                     /label="AmpR promoter"
     terminator      4930..5016
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5108..5135
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"