pMJA289 vector (V015836)

LOCUS       V015836                 9466 bp    DNA     circular SYN 02-FEB-2019
DEFINITION  Exported.
ACCESSION   V015836
VERSION     V015836
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Pscheid R, Wang R, Allegrucci C, Alcocer M.
  TITLE     The intrinsic allergenicity of nut proteins: a lipid story
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9466)
  AUTHORS   Pscheid R, Wang R, Allegrucci C, Alcocer M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2018) School of Biosciences, University of
            Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire
            LE12 5RD, United Kingdom
REFERENCE   3  (bases 1 to 9466)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-AUG-2018) School of Biosciences, University of Nottingham,
            Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD,
            United Kingdom"
FEATURES             Location/Qualifiers
     source          1..9466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             117..750
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    797..922
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1415..1648
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1833..1877
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2026..2067
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2164..2281
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2409..3587
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3655..3996
                     /gene="TRAV10"
                     /label="T cell receptor alpha variable 10"
                     /note="T cell receptor alpha variable 10 from Homo sapiens.
                     Accession#: A0A0B4J240"
     CDS             4063..4482
                     /gene="TRAC"
                     /label="T cell receptor alpha chain constant"
                     /note="T cell receptor alpha chain constant from Homo
                     sapiens. Accession#: P01848"
     misc_feature    4483..4548
                     /label="P2A cleavage sequence"
                     /note="P2A cleavage sequence"
     CDS             4549..5493
                     /gene="TRB"
                     /label="T cell receptor beta chain MC.7.G5"
                     /note="T cell receptor beta chain MC.7.G5 from Homo
                     sapiens. Accession#: P0DTU4"
     misc_feature    5582..6168
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             6188..6784
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             6990..7223
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7380..7604
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        7700..7804
                     /label="AmpR promoter"
     CDS             7805..8662
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8836..9424
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"