Basic Vector Information
- Vector Name:
- pMJA289
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9466 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Pscheid R, Wang R, Allegrucci C, Alcocer M.
- Promoter:
- EF-1α
pMJA289 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMJA289 vector Sequence
LOCUS 62056_16985 9466 bp DNA circular SYN 02-FEB-2019
DEFINITION Cloning vector pMJA289, complete sequence.
ACCESSION MH782475
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9466)
AUTHORS Pscheid R, Wang R, Allegrucci C, Alcocer M.
TITLE The intrinsic allergenicity of nut proteins: a lipid story
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9466)
AUTHORS Pscheid R, Wang R, Allegrucci C, Alcocer M.
TITLE Direct Submission
JOURNAL Submitted (22-AUG-2018) School of Biosciences, University of
Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire
LE12 5RD, United Kingdom
REFERENCE 3 (bases 1 to 9466)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(22-AUG-2018) School of Biosciences, University of Nottingham,
Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD,
United Kingdom"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..9466
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 117..750
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 797..922
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1415..1648
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1833..1877
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 2026..2067
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 2164..2281
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2409..3587
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
CDS 3655..3996
/gene="TRAV10"
/label=TRAV10
/note="T cell receptor alpha variable 10 from Homo sapiens.
Accession#: A0A0B4J240"
CDS 4063..4482
/gene="TRAC"
/label=TRAC
/note="T cell receptor alpha chain constant from Homo
sapiens. Accession#: P01848"
misc_feature 4483..4548
/label=P2A cleavage sequence
/note="P2A cleavage sequence"
CDS 4549..5493
/gene="TRB"
/label=TRB
/note="T cell receptor beta chain MC.7.G5 from Homo
sapiens. Accession#: P0DTU4"
misc_feature 5582..6168
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 6188..6784
/label=PuroR
/note="puromycin N-acetyltransferase"
LTR 6990..7223
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 7380..7604
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
promoter 7700..7804
/label=AmpR promoter
CDS 7805..8662
/label=AmpR
/note="beta-lactamase"
rep_origin 8836..9424
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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