Basic Vector Information
- Vector Name:
- pLX-AS
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4524 bp
- Type:
- Cloning vector
- Replication origin:
- pBBR1 oriV
- Host:
- Plants
- Source/Author:
- Pasin F.
- Promoter:
- CaMV 35S (enhanced)
pLX-AS vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLX-AS vector Sequence
LOCUS 62056_16340 4524 bp DNA circular SYN 17-FEB-2021
DEFINITION Cloning vector pLX-AS, complete sequence.
ACCESSION MW281334
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4524)
AUTHORS Pasin F.
TITLE Oligonucleotide abundance biases aid design of a type IIS synthetic
genomics framework with plant virome capacity
JOURNAL Biotechnol J, e2000354 (2021) In press
PUBMED 33410597
REFERENCE 2 (bases 1 to 4524)
AUTHORS Pasin F.
TITLE Direct Submission
JOURNAL Submitted (19-NOV-2020) Agricultural Biotechnology Research Center,
Academia Sinica, n.a., Taipei, Taipei 11529, Taiwan
REFERENCE 3 (bases 1 to 4524)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
J, e2000354 (2021) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(19-NOV-2020) Agricultural Biotechnology Research Center, Academia
Sinica, n.a., Taipei, Taipei 11529, Taiwan"
FEATURES Location/Qualifiers
source 1..4524
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 25..72
/label=bacterial terminator
/note="bacterial terminator"
/regulatory_class="terminator"
misc_feature 133..157
/label=LB T-DNA repeat
/note="left border repeat from octopine Ach5 T-DNA"
misc_feature 184..208
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
promoter 535..1204
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
misc_feature complement(1208..1214)
/label=AarI
/note="AarI"
CDS complement(1266..1436)
/label=lacZ-alpha
/note="LacZ-alpha fragment of beta-galactosidase"
promoter complement(1485..1515)
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
misc_feature 1551..1557
/label=AarI
/note="AarI"
misc_feature 1556..1562
/label=SapI
/note="SapI"
misc_feature 1562..1565
/label=CCCA (AarI)
/note="CCCA (AarI)"
misc_feature 1564..1566
/label=CAG (SapI)
/note="CAG (SapI)"
terminator 1573..1825
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 1890..1913
/note="RB_pTiA6; RB_pTiA6"
misc_feature 1923..1951
/label=RB_overdrive_pTiA6
/note="RB_overdrive_pTiA6"
regulatory complement(1978..2018)
/label=bacterial terminator
/note="bacterial terminator"
/regulatory_class="terminator"
CDS complement(2065..2877)
/label=KanR
/note="aminoglycoside phosphotransferase"
CDS complement(3006..3665)
/label=pBBR1 Rep
/note="replication protein for the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica"
rep_origin complement(3666..4437)
/direction=LEFT
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
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