pLX-AS vector (V015781)

LOCUS       62056_16340        4524 bp DNA     circular SYN 17-FEB-2021
DEFINITION  Cloning vector pLX-AS, complete sequence.
ACCESSION   MW281334
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Pasin F.
  TITLE     Oligonucleotide abundance biases aid design of a type IIS synthetic 
            genomics framework with plant virome capacity
  JOURNAL   Biotechnol J, e2000354 (2021) In press
  PUBMED    33410597
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2020) Agricultural Biotechnology Research Center, 
            Academia Sinica, n.a., Taipei, Taipei 11529, Taiwan
REFERENCE   3  (bases 1 to 4524)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J, e2000354 (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-NOV-2020) Agricultural Biotechnology Research Center, Academia 
            Sinica, n.a., Taipei, Taipei 11529, Taiwan"
FEATURES             Location/Qualifiers
     source          1..4524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      25..72
                     /label=bacterial terminator
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        535..1204
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(1208..1214)
                     /label=AarI
                     /note="AarI"
     CDS             complement(1266..1436)
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
     promoter        complement(1485..1515)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     misc_feature    1551..1557
                     /label=AarI
                     /note="AarI"
     misc_feature    1556..1562
                     /label=SapI
                     /note="SapI"
     misc_feature    1562..1565
                     /label=CCCA (AarI)
                     /note="CCCA (AarI)"
     misc_feature    1564..1566
                     /label=CAG (SapI)
                     /note="CAG (SapI)"
     terminator      1573..1825
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1890..1913
                     /note="RB_pTiA6; RB_pTiA6"
     misc_feature    1923..1951
                     /label=RB_overdrive_pTiA6
                     /note="RB_overdrive_pTiA6"
     regulatory      complement(1978..2018)
                     /label=bacterial terminator
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     CDS             complement(2065..2877)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(3006..3665)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(3666..4437)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"