Basic Vector Information
- Vector Name:
- pOLK1
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6125 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Sanchez F, Geffroy S, Norest M, Yau S
pOLK1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pOLK1 vector Sequence
LOCUS 62056_18275 6125 bp DNA circular SYN 17-APR-2021
DEFINITION Cloning vector pOLK1, complete sequence.
ACCESSION MW598457
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6125)
AUTHORS Sanchez F, Geffroy S, Norest M, Yau S, Moreau H, Grimsley N.
TITLE Simplified Transformation of Ostreococcus tauri Using Polyethylene
Glycol
JOURNAL Genes (Basel) 10 (5), E399 (2019)
PUBMED 31130696
REFERENCE 2 (bases 1 to 6125)
AUTHORS Sanchez F, Geffroy S, Norest M, Yau S, Moreau H, Grimsley N.
TITLE Direct Submission
JOURNAL Submitted (08-FEB-2021) UMR7232, CNRS, Avenue Pierre Fabre, Banyuls
sur mer 66650, France
REFERENCE 3 (bases 1 to 6125)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genes
(Basel)"; date: "2019"; volume: "10"; issue: "5"; pages: "E399"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(08-FEB-2021) UMR7232, CNRS, Avenue Pierre Fabre, Banyuls sur mer
66650, France"
FEATURES Location/Qualifiers
source 1..6125
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 96..200
/label=AmpR promoter
CDS 201..1058
/label=AmpR
/note="beta-lactamase"
rep_origin 1232..1820
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2108..2129
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2144..2174
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2182..2198
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2206..2222
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 2235..2240
/label=restriction site HindIII
/note="restriction site HindIII"
regulatory 2247..2388
/label=strong stop terminator
/note="strong stop terminator"
/regulatory_class="terminator"
misc_feature 2389..2394
/label=restriction site BglII
/note="restriction site BglII"
regulatory 2395..2708
/label=alpha tubulin promoter
/note="alpha tubulin promoter"
/regulatory_class="promoter"
misc_feature 2587..2592
/label=restriction site XhoI
/note="restriction site XhoI"
misc_feature 2617..2623
/label=restriction site BstEII
/note="restriction site BstEII"
CDS 2712..4361
/label=luciferase
/note="firefly luciferase"
misc_feature 4364..4369
/label=restriction site ClaI
/note="restriction site ClaI"
regulatory 4370..4445
/label=alpha tubulin terminator
/note="alpha tubulin terminator"
/regulatory_class="terminator"
misc_feature 4446..4451
/label=restriction site BamHI
/note="restriction site BamHI"
misc_feature 4450..4455
/label=restriction site SmaI/XmaI
/note="restriction site SmaI/XmaI"
misc_feature 4470..4475
/label=restriction site StuI
/note="restriction site StuI"
misc_feature 4476..4481
/label=restriction site SphI
/note="restriction site SphI"
regulatory 4485..4799
/label=alpha tubulin promoter
/note="alpha tubulin promoter"
/regulatory_class="promoter"
misc_feature 4675..4680
/label=restriction site XhoI
/note="restriction site XhoI"
misc_feature 4705..4711
/label=restriction site BstEII
/note="restriction site BstEII"
CDS 4800..5606
/label=KanR
/note="aminoglycoside phosphotransferase"
regulatory 5615..5690
/label=alpha tubulin terminator
/note="alpha tubulin terminator"
/regulatory_class="terminator"
misc_feature 5696..5701
/label=restriction site XbaI
/note="restriction site XbaI"
misc_feature 5707..5712
/label=restriction site NruI
/note="restriction site NruI"
misc_feature 5713..5718
/label=restriction site KpnI
/note="restriction site KpnI"
misc_feature 5719..5724
/label=multiple cloning site: SacI/SstI
/note="multiple cloning site: SacI/SstI"
primer_bind complement(5731..5747)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 5871..5881
/label=restriction site BglI
/note="restriction site BglI"
misc_feature 5938..5943
/label=restriction site NdeI
/note="restriction site NdeI"
This page is informational only.