pOLK1 vector (V015606)

LOCUS       62056_18275        6125 bp DNA     circular SYN 17-APR-2021
DEFINITION  Cloning vector pOLK1, complete sequence.
ACCESSION   MW598457
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Sanchez F, Geffroy S, Norest M, Yau S, Moreau H, Grimsley N.
  TITLE     Simplified Transformation of Ostreococcus tauri Using Polyethylene 
            Glycol
  JOURNAL   Genes (Basel) 10 (5), E399 (2019)
  PUBMED    31130696
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Sanchez F, Geffroy S, Norest M, Yau S, Moreau H, Grimsley N.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2021) UMR7232, CNRS, Avenue Pierre Fabre, Banyuls 
            sur mer 66650, France
REFERENCE   3  (bases 1 to 6125)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)"; date: "2019"; volume: "10"; issue: "5"; pages: "E399"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-FEB-2021) UMR7232, CNRS, Avenue Pierre Fabre, Banyuls sur mer 
            66650, France"
FEATURES             Location/Qualifiers
     source          1..6125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2235..2240
                     /label=restriction site HindIII
                     /note="restriction site HindIII"
     regulatory      2247..2388
                     /label=strong stop terminator
                     /note="strong stop terminator"
                     /regulatory_class="terminator"
     misc_feature    2389..2394
                     /label=restriction site BglII
                     /note="restriction site BglII"
     regulatory      2395..2708
                     /label=alpha tubulin promoter
                     /note="alpha tubulin promoter"
                     /regulatory_class="promoter"
     misc_feature    2587..2592
                     /label=restriction site XhoI
                     /note="restriction site XhoI"
     misc_feature    2617..2623
                     /label=restriction site BstEII
                     /note="restriction site BstEII"
     CDS             2712..4361
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    4364..4369
                     /label=restriction site ClaI
                     /note="restriction site ClaI"
     regulatory      4370..4445
                     /label=alpha tubulin terminator
                     /note="alpha tubulin terminator"
                     /regulatory_class="terminator"
     misc_feature    4446..4451
                     /label=restriction site BamHI
                     /note="restriction site BamHI"
     misc_feature    4450..4455
                     /label=restriction site SmaI/XmaI
                     /note="restriction site SmaI/XmaI"
     misc_feature    4470..4475
                     /label=restriction site StuI
                     /note="restriction site StuI"
     misc_feature    4476..4481
                     /label=restriction site SphI
                     /note="restriction site SphI"
     regulatory      4485..4799
                     /label=alpha tubulin promoter
                     /note="alpha tubulin promoter"
                     /regulatory_class="promoter"
     misc_feature    4675..4680
                     /label=restriction site XhoI
                     /note="restriction site XhoI"
     misc_feature    4705..4711
                     /label=restriction site BstEII
                     /note="restriction site BstEII"
     CDS             4800..5606
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      5615..5690
                     /label=alpha tubulin terminator
                     /note="alpha tubulin terminator"
                     /regulatory_class="terminator"
     misc_feature    5696..5701
                     /label=restriction site XbaI
                     /note="restriction site XbaI"
     misc_feature    5707..5712
                     /label=restriction site NruI
                     /note="restriction site NruI"
     misc_feature    5713..5718
                     /label=restriction site KpnI
                     /note="restriction site KpnI"
     misc_feature    5719..5724
                     /label=multiple cloning site: SacI/SstI
                     /note="multiple cloning site: SacI/SstI"
     primer_bind     complement(5731..5747)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5871..5881
                     /label=restriction site BglI
                     /note="restriction site BglI"
     misc_feature    5938..5943
                     /label=restriction site NdeI
                     /note="restriction site NdeI"