pSEVA221MBPc-AID-T7RNAP vector (V015579)

LOCUS       V015579                 8899 bp    DNA     circular SYN 06-OCT-2020
DEFINITION  Exported.
ACCESSION   V015579
VERSION     V015579
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Alvarez B, Mencia M, de Lorenzo V, Fernandez LA.
  TITLE     In vivo diversification of target genomic sites using processive T7
            RNA polymerase-base deaminase fusions blocked by RNA-guided dCas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8899)
  AUTHORS   Alvarez B, Fernandez LA.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2020) Microbial Biotechnology, CNB-CSIC (Centro
            Nacional de Biotecnologia-CSIC), Darwin 3, Cantoblanco, Madrid,
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 8899)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-JUL-2020) Microbial Biotechnology, CNB-CSIC (Centro Nacional de
            Biotecnologia-CSIC), Darwin 3, Cantoblanco, Madrid, Madrid 28049,
            Spain"
FEATURES             Location/Qualifiers
     source          1..8899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(32..118)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(164..787)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     promoter        803..858
                     /label="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             895..1995
                     /label="MBP"
                     /note="maltose binding protein from E. coli"
     CDS             2002..2592
                     /label="AID"
                     /note="human activation-induced cytidine deaminase
                     (Dickerson et al., 2003)"
     misc_feature    2599..2682
                     /gene="MBPc-AID-T7RNAP"
                     /label="Linker"
                     /note="Linker"
     CDS             2689..5337
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     terminator      5351..5445
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             5577..6389
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     oriT            6559..6667
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(6731..7876)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     rep_origin      complement(8269..8800)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"