pSKA417 vector (V015577)

LOCUS       62056_19905        4403 bp DNA     circular SYN 26-JUN-2019
DEFINITION  Cloning vector pSKA417, complete sequence.
ACCESSION   MK775703
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Aoki SK, Lillacci G, Gupta A, Baumschlager A, Schweingruber D, 
            Khammash M.
  TITLE     A universal biomolecular integral feedback controller for robust 
            perfect adaptation
  JOURNAL   Nature 570, 533-537 (2019)
  PUBMED    31217585
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Aoki SK, Lillacci G.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2019) Biosystems Science and Engineering, ETH 
            Zurich, Mattenstrasse 26, Basel 4058, Switzerland
REFERENCE   3  (bases 1 to 4403)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature 570,
            533-537 (2019)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-APR-2019) Biosystems Science and Engineering, ETH Zurich, 
            Mattenstrasse 26, Basel 4058, Switzerland"
FEATURES             Location/Qualifiers
     source          1..4403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..25
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      26..31
                     /regulatory_class="minus_35_signal"
     regulatory      32..50
                     /label=TetR tetO2 binding site
                     /note="TetR tetO2 binding site"
                     /regulatory_class="other"
     regulatory      49..54
                     /regulatory_class="minus_10_signal"
     regulatory      67..73
                     /label=sRBS
                     /note="sRBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             80..2443
                     /codon_start=1
                     /transl_table=11
                     /product="mfLon"
                     /label=mfLon
                     /protein_id="QDD67903.1"
                     /translation="MSKKIKLPIFQIRGSFIVPGIKENLEVGRKNTLASVNYAIKNSNN
                     QMIAIPQIDASVEKPEFSDLHEFGILIDFEVIKEWKDNSLTISTNPIQRCKVISFFENE
                     DQVPYAEVELIESINDFSDEELKELIEKISDAIKTKASLVTKQIKQLISGESDDLSLAF
                     DSIMFKLAPSKILTNPEYITSPSLKTRWSIIEKIIFAEDGIITRNAESIDAARQKNEIE
                     QELNHKLKEKMDKQQKEYYLREKMRIIKDELEDEDDSDDSSLEKYKERLAKEPFPEEVK
                     RKIMASIKRVEALQSGTPEWNTEKNYIDWMMSIPWWEETEDLTDLKYAKKILDKHHYGM
                     KKVKERIIEYLAVKTKTKSLKAPIITLVGPPGVGKTSLAKSIAEAVGKNFVKVSLGGVK
                     DESEIRGHRKTYVGSMPGRIIQTMKRAKVKNPLFLLDEIDKMASDHRGDPASAMLEVLD
                     PEQNKEFSDHYIEEPYDLSQVMFIATANYPEDIPEALYDRMEIINLSSYTEIEKVKIAQ
                     DYLVPKAIEQHELTSEEISFTEGAINEIIKYYTREAGVRQLERHINSIIRKYIVKNLNG
                     EMDKIVIDEKQVNDLLGKRIFDHTEKQEESQIGVVTGLAYTQFGGDILPIEVSLYPGKG
                     NLILTGKLGEVMKESATIALTYVKSNFEKFGVDKKVFEENDIHVHVPEGAVPKDGPAAG
                     ITITTALISALSDKPVSKEIGMTGEITLRGNVLPIGGLREKSISASRSGLKTIIIPKKN
                     ERDLDEIPDEVKAKLKIIPAEKYEEVFAIVFKTK"
     terminator      2455..2541
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2711..3256)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(3370..3464)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3479..4267)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"