Basic Vector Information
- Vector Name:
- Hr5-Ie1-tTAV(OX5378)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9633 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Harvey-Samuel TD, Xu X, Lovett E, Dafa'alla T
- Promoter:
- IE1
Hr5-Ie1-tTAV(OX5378) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Hr5-Ie1-tTAV(OX5378) vector Sequence
LOCUS 62056_1216 9633 bp DNA circular SYN 14-MAR-2021
DEFINITION Cloning vector Hr5/Ie1-tTAV(OX5378), complete sequence.
ACCESSION MT533615
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9633)
AUTHORS Harvey-Samuel TD, Xu X, Lovett E, Dafa'alla T, Walker A, Norman VC,
Carter R, Teal J, Akilan L, Leftwich PT, Reitmayer CM, Siddiqui HA,
Alphey L.
TITLE Engineered expression of the invertebrate-specific scorpion toxin
AaHIT reduces adult longevity and female fecundity in the
diamondback moth Plutella xylostella
JOURNAL Pest Manag Sci (2021) In press
PUBMED 33660916
REFERENCE 2 (bases 1 to 9633)
AUTHORS Harvey-Samuel TD.
TITLE Direct Submission
JOURNAL Submitted (27-MAY-2020) Arthropod Genetics, The Pirbright Institute,
Ash Road, Woking, Surrey GU24 0NF, United Kingdom
REFERENCE 3 (bases 1 to 9633)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Pest Manag
Sci (2021) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(27-MAY-2020) Arthropod Genetics, The Pirbright Institute, Ash Road,
Woking, Surrey GU24 0NF, United Kingdom"
FEATURES Location/Qualifiers
source 1..9633
/mol_type="other DNA"
/organism="synthetic DNA construct"
mobile_element 8..673
/mobile_element_type="transposon:Piggybac"
/label=bac
enhancer 744..1226
/label=hr5 enhancer
/note="baculovirus early transcription enhancer"
CDS 2025..2645
/label=TetR
/note="tetracycline repressor TetR"
CDS 2802..3035
/label=VP16 AD
/note="transcriptional activation domain of herpes simplex
virus protein VP16 (Triezenberg et al., 1988; Cousens et
al., 1989)"
regulatory 3487..4027
/regulatory_class="promoter"
CDS 4049..4741
/label=ZsGreen1
/note="Zoanthus green fluorescent protein"
CDS 4760..4780
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 4921..5042
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
mobile_element 5087..6133
/mobile_element_type="transposon:Piggybac"
/label=bac
primer_bind complement(6836..6852)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 7326..7430
/label=AmpR promoter
CDS 7431..8288
/label=AmpR
/note="beta-lactamase"
rep_origin 8462..9050
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 9338..9359
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 9374..9404
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9412..9428
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9436..9452
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
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