Hr5-Ie1-tTAV(OX5378) vector (V015556)

LOCUS       62056_1216        9633 bp DNA     circular SYN 14-MAR-2021
DEFINITION  Cloning vector Hr5/Ie1-tTAV(OX5378), complete sequence.
ACCESSION   MT533615
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Harvey-Samuel TD, Xu X, Lovett E, Dafa'alla T, Walker A, Norman VC, 
            Carter R, Teal J, Akilan L, Leftwich PT, Reitmayer CM, Siddiqui HA, 
            Alphey L.
  TITLE     Engineered expression of the invertebrate-specific scorpion toxin 
            AaHIT reduces adult longevity and female fecundity in the 
            diamondback moth Plutella xylostella
  JOURNAL   Pest Manag Sci (2021) In press
  PUBMED    33660916
REFERENCE   2  (bases 1 to 9633)
  AUTHORS   Harvey-Samuel TD.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2020) Arthropod Genetics, The Pirbright Institute,
            Ash Road, Woking, Surrey GU24 0NF, United Kingdom
REFERENCE   3  (bases 1 to 9633)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pest Manag 
            Sci (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAY-2020) Arthropod Genetics, The Pirbright Institute, Ash Road,
            Woking, Surrey GU24 0NF, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..9633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  8..673
                     /mobile_element_type="transposon:Piggybac"
                     /label=bac
     enhancer        744..1226
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     CDS             2025..2645
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             2802..3035
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
     regulatory      3487..4027
                     /regulatory_class="promoter"
     CDS             4049..4741
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     CDS             4760..4780
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4921..5042
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  5087..6133
                     /mobile_element_type="transposon:Piggybac"
                     /label=bac
     primer_bind     complement(6836..6852)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7326..7430
                     /label=AmpR promoter
     CDS             7431..8288
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8462..9050
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9338..9359
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9374..9404
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9412..9428
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9436..9452
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"