Basic Vector Information
- Vector Name:
- JS_segE_C
- Antibiotic Resistance:
- Kanamycin
- Length:
- 8644 bp
- Type:
- Cloning vector
- Replication origin:
- p15A ori
- Source/Author:
- Shin J.
JS_segE_C vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
JS_segE_C vector Sequence
LOCUS 62056_1365 8644 bp DNA circular SYN 01-APR-2020
DEFINITION Cloning vector JS_segE_C, complete sequence.
ACCESSION MN982376
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8644)
AUTHORS Shin J.
TITLE Programming Escherichia coli to function as a digital display
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8644)
AUTHORS Shin J.
TITLE Direct Submission
JOURNAL Submitted (19-JAN-2020) Biomedical Engineering, Boston University,
44 Cummington Mall, Boston, MA 02215, USA
REFERENCE 3 (bases 1 to 8644)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(19-JAN-2020) Biomedical Engineering, Boston University, 44
Cummington Mall, Boston, MA 02215, USA"
FEATURES Location/Qualifiers
source 1..8644
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(297..842)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
CDS complement(934..1746)
/label=KanR
/note="aminoglycoside phosphotransferase"
regulatory 1903..1984
/label=PSrpR
/note="PSrpR"
/regulatory_class="promoter"
regulatory 1985..2054
/label=PLux2
/note="PLux2"
/regulatory_class="promoter"
misc_feature 2055..2129
/label=RiboJ53
/note="RiboJ53"
regulatory 2130..2147
/label=P3
/note="P3"
/regulatory_class="ribosome_binding_site"
CDS 2148..2750
/codon_start=1
/transl_table=11
/product="PhlF"
/label=PhlF
/protein_id="QIQ56099.1"
/translation="MARTPSRSSIGSLRSPHTHKAILTSTIEILKECGYSGLSIESVAR
RAGASKPTIYRWWTNKAALIAEVYENESEQVRKFPDLGSFKADLDFLLRNLWKVWRETI
CGEAFRCVIAEAQLDPATLTQLKDQFMERRREMPKKLVENAISNGELPKDTNRELLLDM
IFGFCWYRLLTEQLTVEQDIEEFTFLLINGVCPGTQR"
regulatory 2751..2808
/label=ECK120033737
/note="ECK120033737"
/regulatory_class="terminator"
regulatory 2813..2875
/label=PHlyIIR2
/note="PHlyIIR2"
/regulatory_class="promoter"
protein_bind 2896..2914
/label=tet operator
/note="bacterial operator O2 for the tetR and tetA genes"
misc_feature 2950..3024
/label=RiboJ10
/note="RiboJ10"
regulatory 3025..3058
/label=S4
/note="S4"
/regulatory_class="ribosome_binding_site"
CDS 3059..3700
/codon_start=1
/transl_table=11
/product="SrpR"
/label=SrpR
/protein_id="QIQ56100.1"
/translation="MARKTAAEAEETRQRIIDAALEVFVAQGVSDATLDQIARKAGVTR
GAVYWHFNGKLEVLQAVLASRQHPLELDFTPDLGIERSWEAVVVAMLDAVHSPQSKQFS
EILIYQGLDESGLIHNRMVQASDRFLQYIHQVLRHAVTQGELPINLDLQTSIGVFKGLI
TGLLYEGLRSKDQQAQIIKVALGSFWALLREPPRFLLCEEAQIKQVKSFE"
regulatory 3701..3790
/label=ECK120029600
/note="ECK120029600"
/regulatory_class="terminator"
regulatory 3795..4020
/label=PCin
/note="PCin"
/regulatory_class="promoter"
misc_feature 4021..4095
/label=RiboJ51
/note="RiboJ51"
regulatory 4096..4104
/label=H1
/note="H1"
/regulatory_class="ribosome_binding_site"
CDS 4105..4740
/codon_start=1
/transl_table=11
/product="HlyIIR"
/label=HlyIIR
/protein_id="QIQ56101.1"
/translation="MKYILFEVCEMGKSREQTMENILKAAKKKFGERGYEGTSIQEITK
EAKVNVAMASYYFNGKENLYYEVFKKYGLANELPNFLEKNQFNPINALREYLTVFTTHI
KENPEIGTLAYEEIIKESARLEKIKPYFIGSFEQLKEILQEGEKQGVFHFFSINHTIHW
ITSIVLFPKFKKFIDSLGPNETNDTNHEWMPEDLVSRIISALTDKPNI"
regulatory 4741..4800
/label=ECK120010799
/note="ECK120010799"
/regulatory_class="terminator"
regulatory 4847..4900
/label=PLacI
/note="PLacI"
/regulatory_class="promoter"
regulatory 4901..4928
/label=a part of PLacI
/note="a part of PLacI"
/regulatory_class="ribosome_binding_site"
CDS 4929..6008
/label=lacI
/note="lac repressor"
regulatory 6012..6029
/regulatory_class="ribosome_binding_site"
CDS 6030..6650
/label=TetR
/note="tetracycline repressor TetR"
regulatory 6654..6908
/regulatory_class="terminator"
regulatory 6948..6975
/regulatory_class="ribosome_binding_site"
CDS 6976..7725
/gene="luxR"
/label=luxR
/note="Transcriptional activator protein LuxR from
Aliivibrio fischeri. Accession#: P12746"
regulatory 7729..7756
/regulatory_class="ribosome_binding_site"
CDS 7757..8512
/codon_start=1
/transl_table=11
/product="CinR"
/label=CinR
/protein_id="QIQ56105.1"
/translation="MIENTYSEKFESAFEQIKAAANVDAAIRILQAEYNLDFVTYHLAQ
TIASKIDSPFVRTTYPDAWVSRYLLNCYVKVDPIIKQGFERQLPFDWSEVEPTPEAYAM
LVDAQKHGIDDNGYSIPVADKAQRRALLSLNAHIPADEWTELVRRCRNEWIEIAHLIHR
KAVYELHGENDPVPALSPREIECLHWTALGKDYKDISVILGISEHTTRDYLKTARFRLG
CTTISAAASRAVQLRIINPYRIRMTRRNW"
terminator 8524..8595
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 8611..8638
/label=T7Te terminator
/note="phage T7 early transcription terminator"
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