pBRC1-LUC vector (V015480)

LOCUS       62056_4175        7573 bp DNA     circular SYN 20-JAN-2020
DEFINITION  Cloning vector pBRC1-LUC, complete sequence.
ACCESSION   MN728543
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2019) State Key Laboratory of Plant Genomics, 
            Institute of Genetics and Developmental Biology, CAS, West Beichen 
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 7573)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (22-NOV-2019) State Key Laboratory of Plant Genomics, Institute of 
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..7573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..3000
                     /label=promotor of AtBRC1
                     /note="promotor of AtBRC1"
                     /regulatory_class="promoter"
     CDS             3008..4657
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      4678..4930
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4939..4955)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5429..5533
                     /label=AmpR promoter
     CDS             5534..6391
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6565..7153
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7441..7462
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7477..7507
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7515..7531
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7539..7555
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"