pCrRNA-V vector (V015472)

LOCUS       62056_7980        5214 bp DNA     circular SYN 17-MAY-2021
DEFINITION  Cloning vector pCrRNA-V, complete sequence.
ACCESSION   MW285724
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Lin Z, Li H, He L, Jing Y, Pistolozzi M, Wang T, Ye Y.
  TITLE     Efficient genome editing for Pseudomonas aeruginosa using 
            CRISPR-Cas12a
  JOURNAL   Gene, 145693 (2021) In press
  PUBMED    33961975
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Lin Z, Li H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2020) School of Biology and Biological 
            Engineering, South China University of Technology, 382 East Outer 
            Loop Road, University Park, Guangzhou, Guangdong 510006, China
REFERENCE   3  (bases 1 to 5214)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene, 
            145693 (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-NOV-2020) School of Biology and Biological Engineering, South 
            China University of Technology, 382 East Outer Loop Road, University
            Park, Guangzhou, Guangdong 510006, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..301
                     /regulatory_class="promoter"
     CDS             302..1720
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     terminator      1747..1841
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1872..1900
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2089..2619
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     oriT            2833..2941
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      3035..3623
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3680..4510)
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      4524..4875
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(4948..5034)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        5071..5105
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     misc_feature    5106..5124
                     /label=5' handle with the crRNA
                     /note="5' handle with the crRNA"
     regulatory      5147..5153
                     /regulatory_class="terminator"