pKO403-lacZ-P15A vector (V015460)

LOCUS       62056_13535        4591 bp DNA     circular SYN 01-SEP-2021
DEFINITION  Bifidobacterium-E. coli shuttle vector pKO403-lacZ-P15A DNA, 
            complete sequence.
ACCESSION   LC647193
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Hend A, Yuka O, Tomoya K, Kouta S, Izumi N, Tohru S.
  TITLE     Bifidobacterium-E. coli shuttle vector series, pKO403, for metabolic
            engineering
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Hend A, Yuka O, Tomoya K, Kouta S, Izumi N, Tohru S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2021) Contact:Altaib Hend Gifu University, Applied
            Biological Science; 1-1Yanagido, Gifu, Gifu 501-1193, Japan
REFERENCE   3  (bases 1 to 4591)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-AUG-2021) Contact:Altaib Hend Gifu University, Applied 
            Biological Science; 1-1Yanagido, Gifu, Gifu 501-1193, Japan"
FEATURES             Location/Qualifiers
     source          1..4591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..11
                     /label=sapI site
                     /note="sapI site"
     primer_bind     334..350
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(354..410)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(420..436)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(444..460)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(468..498)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(513..534)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    638..648
                     /label=sapI site
                     /note="sapI site"
     misc_feature    662..2286
                     /label=Ori-Ts
                     /note="Ori-Ts"
     gene            1288..1978
                     /gene="repB"
                     /label=repB
     misc_feature    1288..1978
                     /gene="repB"
     misc_feature    2301..2534
                     /label=hup terminator
                     /note="hup terminator"
     rep_origin      complement(2833..3378)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     misc_feature    3448..4550
                     /label=spectinomycin resistance gene
                     /note="spectinomycin resistance gene"