Basic Vector Information
- Vector Name:
- pMM7-10-1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9544 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Das SR, Maselko MB, Upadhyay A, Smanski MJ.
pMM7-10-1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMM7-10-1 vector Sequence
LOCUS 62056_17165 9544 bp DNA circular SYN 05-AUG-2020
DEFINITION Cloning vector pMM7-10-1, complete sequence.
ACCESSION MN630871
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9544)
AUTHORS Das SR, Maselko MB, Upadhyay A, Smanski MJ.
TITLE Batch cultivation of non-transgenic males suitable for juvenile
release SIT programs
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9544)
AUTHORS Das SR, Maselko MB, Upadhyay A, Smanski MJ.
TITLE Direct Submission
JOURNAL Submitted (30-OCT-2019) Biotechnology Institute, University of
Minnesota, 1479 Gortner Ave, St Paul, MN 55108, USA
REFERENCE 3 (bases 1 to 9544)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(30-OCT-2019) Biotechnology Institute, University of Minnesota, 1479
Gortner Ave, St Paul, MN 55108, USA"
FEATURES Location/Qualifiers
source 1..9544
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter complement(24..42)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(49..65)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(206..661)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 688..792
/label=AmpR promoter
CDS 793..1650
/label=AmpR
/note="beta-lactamase"
rep_origin 1824..2412
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2700..2721
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2736..2766
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2774..2790
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2798..2814
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 2835..2853
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
protein_bind complement(3072..3141)
/label=attB
/note="attB site for the phi-C31 integrase (Groth et al.,
2000)"
protein_bind complement(3685..3963)
/label=tetracycline response element
/note="contains seven copies of the tetracycline operator
tetO"
regulatory 3983..4135
/label=Hsp70
/note="Hsp70"
/regulatory_class="promoter"
intron 4146..4212
/label=Mhc intron
/note="modified Drosophila myosin heavy chain intron 16
(Pfeiffer et al., 2010)"
5'UTR 4234..4254
/label=syn21 5'UTR
/note="syn21 5'UTR"
CDS 4255..4878
/label=TetR
/note="tetracycline repressor TetR"
CDS 5035..5268
/label=VP16 AD
/note="transcriptional activation domain of herpes simplex
virus protein VP16 (Triezenberg et al., 1988; Cousens et
al., 1989)"
intron 5356..5421
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 5551..5571
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 5996..6128
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 6396..8204
/label=Polyubiquitin
/note="Promoter of polyubiqutin gene of Drosophila
melanogaster"
CDS 8229..8249
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 8433..9149
/label=EGFP
/note="enhanced GFP"
misc_feature 9153..9393
/label=terminator
/note="terminator"
promoter complement(9462..9480)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 9511..9529
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
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