pD2 vector (V015405)

LOCUS       62056_8215        3844 bp DNA     circular SYN 14-NOV-2020
DEFINITION  Cloning vector pD2, complete sequence.
ACCESSION   MT180326
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Luo M, Xie L, Chakraborty S, Wang A, Matny O, Richardson T, Bhatt D,
            Hoque M, Patpour M, Sorenson C, Steuernagel B, Wulff B, Upadhyaya N,
            Mago R, Periyannan S, Lagudah E, Freedman R, Reuber L, Steffenson B,
            Ayliffe M.
  TITLE     A wheat stem rust resistance multi-transgene cassette provides high 
            levels of field protection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Luo M, Xie L, Chakraborty S, Wang A, Matny O, Richardson T, Bhatt D,
            Hoque M, Patpour M, Sorenson C, Steuernagel B, Wulff B, Upadhyaya N,
            Mago R, Periyannan S, Lagudah E, Freedman R, Reuber L, Steffenson B,
            Ayliffe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2020) RP1, CSIRO A
REFERENCE   3  (bases 1 to 3844)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-MAR-2020) RP1, CSIRO A"
FEATURES             Location/Qualifiers
     source          1..3844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    33..132
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    259..280
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        295..325
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    333..349
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     357..373
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(398..414)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    671..902
                     /label=attP1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    904..921
                     /label=I-Sce I
                     /note="I-Sce I"
     promoter        complement(924..942)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1206..1310
                     /label=AmpR promoter
     CDS             1311..2168
                     /label=AmpR
                     /note="beta-lactamase"
     protein_bind    complement(2267..2300)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2334..2458)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    2460..2578
                     /label=multiple cloning site
                     /note="multiple cloning site"
     protein_bind    complement(2579..2674)
                     /label=attL3
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    2677..2713
                     /label=PI-Sce I
                     /note="PI-Sce I"
     rep_origin      2795..3383
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3569..3709)
                     /label=bom
                     /note="basis of mobility region from pBR322"