MyoUTipGAPi vector (V015368)

MSDS

Basic Vector Information

Vector Name:: MyoUTipGAPi

Antibiotic Resistance:: Ampicillin

Length:: 9575 bp

Type:: UNVERIFIED: Cloning vector

Replication origin:: ori

Source/Author:: Foley SJ, Orr RG, Galotto G, Liu B

Promoter:: Ubi

MyoUTipGAPi vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

MyoUTipGAPi vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_1725        9575 bp DNA     circular SYN 25-MAY-2020
DEFINITION  UNVERIFIED: Cloning vector MyoUTipGAPi, complete sequence.
ACCESSION   MK975252
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Foley SJ, Orr RG, Galotto G, Liu B, Vidali L.
  TITLE     Robust survival-based RNAi in plants using in tandem silencing of 
            adenine phosphoribosyltransferase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9575)
  AUTHORS   Foley SJ, Orr RG, Galotto G, Liu B, Vidali L.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2019) Biology and Biotechnology, Worcester 
            Polytechnic Institute, 100 Institute Rd., Worcester, MA 01609, USA
REFERENCE   3  (bases 1 to 9575)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-2019) Biology and Biotechnology, Worcester Polytechnic 
            Institute, 100 Institute Rd., Worcester, MA 01609, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
            GenBank staff is unable to verify sequence and/or annotation 
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..9575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2389..2413
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3237..3261)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3652..3676
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4500..4524)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4943..5195
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5228..5572
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5602..6624
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6696..6872
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7429..7445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7453..7469)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7477..7507)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7522..7543)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7831..8419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8593..9450)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9451..9555)
                     /label=AmpR promoter
     promoter        9575
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"

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