pIgNAT vector (V015352)

LOCUS       62056_12835        6272 bp DNA     circular SYN 08-APR-2020
DEFINITION  Expression vector pIgNAT, complete sequence.
ACCESSION   MK903009
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Balestreri C, Stern RF, Wheeler G, Brownlee C, Highfield A.
  TITLE     pIgNAT, a nourseothricin resistance construct to detect 
            transformation capabilities in the alga, Isochryis galbana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Faktorova D, Nisbet E, Jose FR, Casacuberta E, Allen AE.
  TITLE     Genetic tool development in marine protists: Emerging model 
            organisms for experimental cell biology
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6272)
  AUTHORS   Balestreri C, Stern RF, Wheeler G, Brownlee C, Highfield A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2019) CPR survey, Marine Biological Association, 
            Citadel Hill, Plymouth PL1 2PB, United Kingdom
REFERENCE   4  (bases 1 to 6272)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-2019) CPR survey, Marine Biological Association, Citadel 
            Hill, Plymouth PL1 2PB, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..6272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     3'UTR           complement(402..1560)
                     /gene="Hsp70"
                     /label=derived from Emiliania huxleyi
                     /note="derived from Emiliania huxleyi"
     CDS             complement(1564..2124)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     5'UTR           complement(2137..4002)
                     /gene="Hsp70"
                     /label=derived from Emiliania huxleyi
                     /note="derived from Emiliania huxleyi"
     primer_bind     complement(4051..4067)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4075..4091)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4099..4129)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4144..4165)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4453..5041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5215..6072)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6073..6177)
                     /label=AmpR promoter