Basic Vector Information
- Vector Name:
- pMSCV-syn-PSD95.FingR-GFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7231 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Bensussen S, Shankar S, Ching KH, Zemel D
- Promoter:
- MSCV
pMSCV-syn-PSD95.FingR-GFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMSCV-syn-PSD95.FingR-GFP vector Sequence
LOCUS 62056_17335 7231 bp DNA circular SYN 27-JUN-2020
DEFINITION Cloning vector pMSCV-syn-PSD95.FingR-GFP, complete sequence.
ACCESSION MT612433
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7231)
AUTHORS Bensussen S, Shankar S, Ching KH, Zemel D, Ta TL, Mount RA, Shroff
SN, Gritton HJ, Fabris P, Vanbenschoten H, Beck C, Man H-Y., Han X.
TITLE A viral toolbox of genetically encoded fluorescent synaptic tags
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7231)
AUTHORS Bensussen S, Shankar S, Ching KH, Zemel D, Ta TL, Mount RA, Shroff
SN, Gritton HJ, Fabris P, Vanbenschoten H, Beck C, Man H-Y., Han X.
TITLE Direct Submission
JOURNAL Submitted (15-JUN-2020) Biomedical Engineering, Boston University,
44 Cummington Mall, Boston, MA 02215, USA
REFERENCE 3 (bases 1 to 7231)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-JUN-2020) Biomedical Engineering, Boston University, 44
Cummington Mall, Boston, MA 02215, USA"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7231
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 342..517
/label=5' LTR (truncated)
/note="truncated long terminal repeat from Moloney murine
sarcoma virus"
misc_feature 581..922
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 989..1405
/codon_start=1
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
/translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
promoter 1445..1892
/label=hSyn promoter
/note="human synapsin I promoter; confers neuron-specific
expression (Kugler et al., 2003)"
CDS 2202..2225
/codon_start=1
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/translation="DYKDDDDK"
CDS 2250..2960
/codon_start=1
/label=EGFP
/note="enhanced GFP"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELY"
misc_feature 2976..3553
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 3592..4107
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
primer_bind complement(4276..4292)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4300..4316)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4324..4354)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4369..4390)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4678..5266)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5440..6297)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6298..6402)
/label=AmpR promoter
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