tetO-AaHIT(AGG1074) vector (V015320)

LOCUS       62056_23420        8763 bp DNA     circular SYN 14-MAR-2021
DEFINITION  Cloning vector tetO-AaHIT(AGG1074), complete sequence.
ACCESSION   MT533613
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Harvey-Samuel TD, Xu X, Lovett E, Dafa'alla T, Walker A, Norman VC, 
            Carter R, Teal J, Akilan L, Leftwich PT, Reitmayer CM, Siddiqui HA, 
            Alphey L.
  TITLE     Engineered expression of the invertebrate-specific scorpion toxin 
            AaHIT reduces adult longevity and female fecundity in the 
            diamondback moth Plutella xylostella
  JOURNAL   Pest Manag Sci (2021) In press
  PUBMED    33660916
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   Harvey-Samuel TD.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2020) Arthropod Genetics, The Pirbright Institute,
            Ash Road, Woking, Surrey GU24 0NF, United Kingdom
REFERENCE   3  (bases 1 to 8763)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pest Manag 
            Sci (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAY-2020) Arthropod Genetics, The Pirbright Institute, Ash Road,
            Woking, Surrey GU24 0NF, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..8763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     208..267
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
     mobile_element  698..1370
                     /mobile_element_type="transposon:Piggybac"
                     /label=bac
     primer_bind     complement(1523..1539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1547..1563)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1571..1601)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1616..1637)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1925..2513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2687..3544)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3545..3649)
                     /label=AmpR promoter
     primer_bind     4123..4139
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  4842..5888
                     /mobile_element_type="transposon:Piggybac"
                     /label=bac
     enhancer        6005..6487
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     CDS             7334..8008
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     CDS             8018..8038
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    8179..8300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8345..8615)
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"