Basic Vector Information
- Vector Name:
- Hym-176E_EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5639 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Noro Y, Yum S, Nishimiya-Fujisawa C, Busse C
Hym-176E_EGFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Hym-176E_EGFP vector Sequence
LOCUS 62056_1291 5639 bp DNA circular SYN 23-JAN-2019
DEFINITION Expression vector Hym-176E_EGFP DNA, complete sequence.
ACCESSION LC426376
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5639)
AUTHORS Noro Y, Yum S, Nishimiya-Fujisawa C, Busse C, Shimizu H, Mineta K,
Zhang X, Holstein TW, David CN, Gojobori T, Fujisawa T.
TITLE Regionalized nervous system in Hydra and the mechanism of its
development
JOURNAL Gene Expr. Patterns (2019) In press
REFERENCE 2 (bases 1 to 5639)
AUTHORS Noro Y, Fujisawa T, Shimizu H.
TITLE Direct Submission
JOURNAL Submitted (26-SEP-2018) Contact:Yukihiko Noro Waseda University,
Center for Advanced Biomedical Sciences (TWIns); 2-2 Wakamatsu-cho,
Shinjuku-ku, Tokyo 162-8480, Japan
REFERENCE 3 (bases 1 to 5639)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene Expr.
Patterns (2019) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(26-SEP-2018) Contact:Yukihiko Noro Waseda University, Center for
Advanced Biomedical Sciences (TWIns); 2-2 Wakamatsu-cho,
Shinjuku-ku, Tokyo 162-8480, Japan"
FEATURES Location/Qualifiers
source 1..5639
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 362..466
/label=AmpR promoter
CDS 467..1324
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1498..2086
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2374..2395
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2410..2440
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2448..2464
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2472..2488
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 4399..5112
/codon_start=1
/label=GFP
/note="Aequorea victoria green fluorescent protein"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
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