pS438D.colE3 vector (V015273)

LOCUS       V015273                 8244 bp    DNA     circular SYN 26-NOV-2019
DEFINITION  Exported.
ACCESSION   V015273
VERSION     V015273
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Calles B, Goni-Moreno A, de Lorenzo V.
  TITLE     Digitalizing heterologous gene expression in Gram-negative bacteria
            with a portable on/off module
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8244)
  AUTHORS   Calles B, Goni-Moreno A, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2019) Systems Biology Program, Centro Nacional de
            Biotecnologia, Darwin, 3, Cantoblanco, Madrid 28049, Spain
REFERENCE   3  (bases 1 to 8244)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-AUG-2019) Systems Biology Program, Centro Nacional de
            Biotecnologia, Darwin, 3, Cantoblanco, Madrid 28049, Spain"
FEATURES             Location/Qualifiers
     source          1..8244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(22..984)
                     /label="XylS"
                     /note="XylS regulator encoded by the Pseudomonas putida TOL
                     plasmid pWWO"
     promoter        1884..1964
                     /label="Pm promoter"
                     /note="The bacterial Pm promoter is activated by XylS in
                     the presence of benzoate or m-toluate (Marques et al.,
                     1999)."
     regulatory      1999..2005
                     /regulatory_class="ribosome_binding_site"
     CDS             2017..3096
                     /label="lacI"
                     /note="lac repressor"
     CDS             3097..3114
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             3140..4789
                     /gene="ceaC"
                     /label="Colicin-E3"
                     /note="Colicin-E3 from Escherichia coli. Accession#:
                     P00646"
     misc_feature    4793..4849
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     regulatory      4882..4911
                     /label="synthetic T500 terminator"
                     /note="synthetic T500 terminator"
                     /regulatory_class="terminator"
     terminator      complement(4918..4945)
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     terminator      4985..5028
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      5046..5154
                     /note="anti LacI sRNA; MicC-based"
                     /regulatory_class="other"
     regulatory      complement(5155..5206)
                     /label="PA1/O4s"
                     /note="PA1/O4s"
                     /regulatory_class="promoter"
     terminator      5217..5311
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             5547..6335
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     oriT            6484..6592
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(6606..7265)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(7266..8037)
                     /direction=LEFT
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     terminator      complement(8152..8238)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"